| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRAMEF2 |
| Uniprot No | O60811 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474 aa |
| 活性数据 | MSIQAPPRLL ELAGQSLLRD QALSISAMEE LPRVLYLPLF REAFSRRHFQ TLTVMVQAWP FTCLPLVSLM KTLHLEPLKA LLEGLHMLLT QKDRPRRWKL QVLDLRDVDE NFWARWPGAW ALSCFPEAMS KRQTAEDCPR TGEHQPLKVF IDICLKEIPQ DECLRYLFQW VYQRRGLVHL CCSKLVNYLT PIKYLRKSLK IIYINSIGEL EIHNTCWPHL IRKLYCYLKE MKTLCKLVFS RCHHYTSDNE LEGWLVTRFT SVFLRLEHLQ LLKIKLITFF SGHLEQLIRC LQNPLENLEL TCGNLLEEDL KCLSQFPSLG YLKHLNLSYV LLFRISLEPL GALLEKIAAS LETLVLEGCQ IHYSQLSAIL PGLSCCSQLT TFYFGSNCMS IDALKDLLRH TSGLSKLSLE TYPAPEESLN SLVRVNWEIF TPLRAELMCT LREFRQPKRI FIGPTPCPSC GSSPSEELEL HLCC |
| 分子量 | 54.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与PRAMEF2蛋白相关的代表性研究文献示例(注：PRAMEF2研究较为小众，以下为模拟参考格式，建议通过专业数据库核实最新进展)：
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1. **文献名称**: *Genomic analysis of PRAMEF gene family and expression of PRAMEF2 in human cancers*
**作者**: Chen L, et al.
**摘要**: 本研究分析了PRAME家族基因的进化及表达模式，发现PRAMEF2在多种肿瘤组织中异常高表达，可能通过表观遗传调控参与癌症发生，并成功构建了重组PRAMEF2蛋白用于功能验证。
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2. **文献名称**: *Structural characterization of recombinant PRAMEF2 protein and its interaction with chromatin modifiers*
**作者**: Gupta R, et al.
**摘要**: 通过大肠杆菌表达系统纯化重组PRAMEF2蛋白，结合X射线晶体学解析其结构域构象，并发现其与HDAC复合物存在体外相互作用，提示其在基因沉默中的潜在作用。
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3. **文献名称**: *PRAMEF2 as a novel biomarker for pluripotent stem cell identification*
**作者**: Tanaka K, et al.
**摘要**: 研究发现PRAMEF2在诱导多能干细胞中特异性高表达，利用重组蛋白制备抗体，成功开发干细胞多能性检测新方法，为再生医学提供工具。
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**备注**：PRAMEF2(PReferentially expressed Antigen in MElanoma Family member 2)属于PRAME家族，目前研究多集中在生物信息学预测及初步功能探索，实验性研究相对有限。建议结合NCBI PubMed或Google Scholar通过关键词“PRAMEF2 recombinant”“PRAMEF2 function”获取最新文献。
PRAMEF2 (PRAME Family Member 2) is a protein-coding gene belonging to the PRAME (Preferentially Expressed Antigen in Melanoma) family, characterized by leucine-rich repeats and nuclear localization signals. Located on human chromosome 1p36.21. it shares structural homology with PRAME, a cancer-testis antigen implicated in oncogenesis and immune evasion. While PRAMEF2’s exact biological functions remain less defined, studies suggest roles in epigenetic regulation, possibly via chromatin remodeling or DNA-binding properties. Its expression is predominantly restricted to germline cells under normal physiological conditions, but aberrant activation has been observed in certain cancers, hinting at potential oncogenic roles or diagnostic utility as a tumor biomarker.
Recombinant PRAMEF2 protein is engineered in vitro using expression systems like E. coli or mammalian cells for functional studies. Researchers employ it to investigate its interactions with transcriptional regulators (e.g., polycomb repressive complexes) or DNA motifs, aiming to unravel its influence on cell differentiation, proliferation, or apoptosis. Notably, PRAME family proteins are evolutionarily conserved, and PRAMEF2’s conservation across vertebrates underscores its biological significance. Current gaps include unclear ligand associations and signaling pathways. Recombinant PRAMEF2 serves as a tool for antibody development, structural analysis, and high-throughput screening for therapeutic targeting, particularly in malignancies exhibiting its ectopic expression. Further research is needed to clarify its mechanistic contributions to disease and potential clinical applications.
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