| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRAMEF12 |
| Uniprot No | O95522 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483 aa |
| 活性数据 | MSLQAPPRLL ELAEQSLLRD RALAIPTLEE LPRELFPPLF MEAFTRRCCE TLTTMVQAWP FTCLPLGSLM KSCNLEIFRA VLEGLDALLA QKVRPRRWKL QVLDLRNVDE NFWGIWSGAS ALSPEALSKR RTAGNCPRPG GQQPLMVILD LCFKNGMLDE CLTHFLEWGK QRKGLLHVCC KELQIFGIAI HRIIEVLNTV ELDCIQEVEV CCPWELSILI RFAPYLGQMR NLRKLVLFNI HVSACIPLDR KEQFVIQFTS QFLKLDYFQK LYMHSVSFLE GHLDQLLRCL QAPLETVVMT ECLLSESDLK HLSWCPSIRQ LKELDLRGIT LTHFSPEPLS VLLEQAEATL QTLDLEDCGI VDSQLSAILP ALSRCSQLST FSFCGNLISM AALENLLRHT VGLSKLSLEL YPAPLESYDA QGALCWGRFS QLGAELMKTL RDLRQPKIIV FSTVPCPRCG IRASYDLEPS HCLLNACCQG GFI |
| 分子量 | 54.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRAMEF12蛋白的**虚构参考文献示例**(实际文献可能有限，建议通过专业数据库核实)：
1. **文献名称**：*PRAMEF12: A Novel Member of the PRAME Family with Epigenetic Regulatory Functions*
**作者**：Smith A, et al.
**摘要**：本研究首次报道了PRAMEF12蛋白的重组表达及功能分析。通过大肠杆菌系统成功表达并纯化重组人PRAMEF12蛋白，发现其通过结合组蛋白修饰酶调控靶基因沉默，可能在生殖细胞发育和癌症中发挥作用。
2. **文献名称**：*Structural Characterization of Recombinant PRAMEF12 and Its Interaction with Chromatin*
**作者**：Chen L, et al.
**摘要**：利用X射线晶体学解析了重组PRAMEF12蛋白的结构，揭示其N端保守结构域与染色质重塑复合体的相互作用，为PRAME家族蛋白在表观遗传调控中的机制提供了新见解。
3. **文献名称**：*PRAMEF12 Overexpression Promotes Tumor Cell Proliferation via MAPK Signaling*
**作者**：Wang Y, et al.
**摘要**：在肺癌细胞中重组表达PRAMEF12蛋白，证实其通过激活MAPK信号通路促进细胞增殖，提示其作为癌症治疗靶点的潜在价值。
4. **文献名称**：*Developmental Stage-Specific Expression of PRAMEF12 in Mammalian Testis*
**作者**：Kimura R, et al.
**摘要**：通过重组PRAMEF12蛋白的抗体检测发现，其在哺乳动物睾丸生殖细胞中特异性高表达，推测其参与减数分裂和精子形成调控。
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**注意**：以上为模拟内容，实际研究中关于PRAMEF12的文献较为稀缺。建议通过**PubMed**或**Web of Science**以关键词“PRAMEF12 recombinant”或“PRAME family”检索最新研究，或关注相关表观遗传学及癌症生物学领域的论文。
PRAMEF12 (PReferentially expressed Antigen in MElanoma Family Member 12) is a human protein encoded by the _PRAMEF12_ gene, located on chromosome 1p36.21. It belongs to the PRAME family, a group of cancer-testis antigens (CTAs) primarily expressed in germline cells and aberrantly reactivated in various malignancies. While PRAMEF12’s exact biological function remains unclear, it shares conserved structural features with other PRAME proteins, including a leucine-rich repeat (LRR) domain, suggesting potential roles in protein-protein interactions or immune regulation.
Recombinant PRAMEF12 protein is synthesized using biotechnological platforms (e.g., bacterial or mammalian expression systems) for functional studies. Its restricted expression pattern in normal tissues and re-expression in cancers have sparked interest in its diagnostic or immunotherapeutic potential. However, research on PRAMEF12 is limited compared to well-characterized family members like PRAME, which is implicated in retinoic acid signaling and cancer progression.
Current studies focus on elucidating its involvement in cellular processes such as epigenetic regulation, apoptosis, or immune evasion. Recombinant PRAMEF12 serves as a tool to explore its interaction networks, structural biology, and possible autoantibody responses in cancer. Further characterization could uncover its role in oncogenesis or germ cell development, offering avenues for targeted therapies or biomarkers. Nevertheless, functional validation and clinical relevance require deeper investigation.
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