| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRAF2 |
| Uniprot No | O60831 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-178 aa |
| 活性数据 | MSEVRLPPLRALDDFVLGSARLAAPDPCDPQRWCHRVINNLLYYQTNYLLCFGIGLALAGYVRPLHTLLSALVVAVALGVLVWAAETRAAVRRCRRSHPAACLAAVLAVGLLVLWVAGGACTFLFSIAGPVLLILVHASLRLRNLKNKIENKIESIGLKRTPMGLLLEALGQEQEAGS |
| 分子量 | 45.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于具体关于重组人PRAF2蛋白的文献较为有限，以下提供**示例性参考文献**，供参考格式和内容方向。建议通过学术数据库(如PubMed、Google Scholar)进一步检索最新研究。
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1. **文献名称**：*Recombinant Expression and Functional Analysis of Human PRAF2 in Apoptosis Regulation*
**作者**：Zhang et al.
**摘要**：研究通过大肠杆菌系统表达重组人PRAF2蛋白，证实其通过调控Bcl-2家族蛋白活性促进肿瘤细胞凋亡，为癌症治疗提供潜在靶点。
2. **文献名称**：*PRAF2 Overexpression in Hepatocellular Carcinoma: Role of Recombinant Protein in Cell Proliferation*
**作者**：Li et al.
**摘要**：利用哺乳动物细胞表达的重组PRAF2蛋白，揭示了其在肝癌细胞中通过激活ERK信号通路促进增殖，并与患者不良预后相关。
3. **文献名称**：*Structural Insights into PRAF2-Rab GTPase Interaction via Recombinant Protein Crystallography*
**作者**：Chen et al.
**摘要**：解析重组人PRAF2蛋白的晶体结构，阐明其与Rab3A的结合模式，为神经递质运输机制提供分子基础。
4. **文献名称**：*PRAF2 Modulates Chemoresistance in Leukemia: Evidence from Recombinant Protein-Based Assays*
**作者**：Wang et al.
**摘要**：通过体外重组蛋白实验，发现PRAF2通过抑制caspase-3激活增强白血病细胞对化疗药物的耐药性。
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**注意**：以上为示例文献，实际研究需通过学术数据库验证。检索建议关键词：`"recombinant PRAF2"`、`"PRAF2 protein function"`、`"PRAF2 expression"`。
**Background of Recombinant Human PRAF2 Protein**
The PRAF2 (Prenylated Rab Acceptor Protein Family Member 2) is a conserved eukaryotic protein encoded by the *PRAF2* gene, located on human chromosome Xp11.23. Belonging to the PRAF protein family, it shares structural homology with other members (PRAF1. PRAF3) but exhibits distinct cellular roles. PRAF2 is characterized by four transmembrane domains and participates in intracellular vesicular trafficking, primarily by interacting with Rab GTPases, key regulators of membrane trafficking. It localizes to the Golgi apparatus and endoplasmic reticulum, influencing organelle dynamics and protein transport.
Recombinant human PRAF2 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling studies on its structure and function. PRAF2 has garnered attention for its dual roles in apoptosis regulation and cancer progression. It promotes apoptosis by interacting with pro-apoptotic proteins like Bcl-2 family members, yet paradoxically, its overexpression correlates with poor prognosis in cancers such as neuroblastoma and hepatocellular carcinoma, suggesting context-dependent roles.
Additionally, PRAF2 is implicated in neurological disorders. It interacts with γ-secretase, modulating amyloid-beta production in Alzheimer’s disease models. Dysregulation of PRAF2 is also linked to X-linked intellectual disability, highlighting its importance in neuronal development.
Research on recombinant PRAF2 focuses on elucidating its molecular mechanisms, therapeutic targeting in cancers, and its role in neurodegenerative diseases. Its study aids in understanding membrane trafficking networks and disease pathogenesis, positioning PRAF2 as a potential biomarker or drug target.
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