| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PQLC3 |
| Uniprot No | Q8N755 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-202 aa |
| 活性数据 | MEAALLGLCNWSTLGVCAALKLPQISAVLAARSARGLSLPSLLLELAGFLVFLRYQCYYGYPPLTYLEYPILIAQDVILLLCIFHFNGNVKQATPYIAVLVSSWFILALQKWIIDLAMNLCTFISAASKFAQLQCLWKTRDSGTVSALTWSLSSYTCATRIITTLMTTNDFTILLRFVIMLALNIWVTVTVLRYRKTAIKAE |
| 分子量 | 49 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于PQLC3相关研究的虚构参考文献示例(实际文献可能需要通过学术数据库检索确认)：
1. **"Recombinant human PQLC3 facilitates lysosomal amino acid transport"**
*作者：Chen et al., 2021*
**摘要**：研究通过重组表达人PQLC3蛋白，证实其在HEK293细胞中促进溶酶体胱氨酸转运的功能，并解析其与溶酶体膜蛋白V-ATPase的相互作用。
2. **"Structural characterization of human PQLC3 using cryo-EM"**
*作者：Wang & Liu, 2020*
**摘要**：利用冷冻电镜技术解析重组PQLC3蛋白的三维结构，揭示其保守的PQ-loop结构域在底物识别中的作用，为溶酶体贮积症治疗提供结构基础。
3. **"PQLC2 and PQLC3: Differential roles in mTORC1 signaling"**
*作者：Kim et al., 2019*
**摘要**：比较PQLC2和PQLC3在调控mTORC1通路中的功能差异，发现重组PQLC3通过感知溶酶体亮氨酸水平间接影响细胞生长调控。
4. **"Expression and purification of functional PQLC3 in E. coli"**
*作者：Zhang et al., 2018*
**摘要**：开发了一种在大肠杆菌中高效表达可溶性重组人PQLC3蛋白的方案，并通过体外实验验证其结合精氨酸的能力。
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**注**：以上文献为示例性质，实际研究需参考PubMed、Google Scholar等平台的真实论文。若需具体文献，建议补充蛋白功能关键词(如溶酶体、氨基酸转运)进行精准检索。
PQLC3 (PQ loop repeat-containing protein 3) is a lysosome-targeting transmembrane protein belonging to the PQ-loop protein family, characterized by conserved proline-glutamine (PQ) motifs. It plays a critical role in cellular amino acid homeostasis, particularly in lysosomal cationic amino acid (e.g., arginine, lysine) transport. Studies suggest PQLC3 facilitates the export of these amino acids from lysosomes to the cytosol, integrating nutrient sensing with cellular metabolism via pathways like mTORC1 signaling. Its dysfunction is linked to lysosomal storage disorders and neurodegenerative conditions. Structurally, PQLC3 contains five transmembrane domains and conserved PQ motifs critical for substrate binding. Recombinant human PQLC3 is often produced in E. coli or mammalian expression systems for functional studies, enabling exploration of its role in lysosomal biology, autophagy, and disease mechanisms. Recent research highlights its potential as a therapeutic target for metabolic or neurological diseases involving lysosomal dysregulation. Structural analyses using X-ray crystallography or cryo-EM are ongoing to elucidate its transport mechanisms.
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