| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP4R1L |
| Uniprot No | Q9P1A2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415 aa |
| 活性数据 | MAEIPLYFVD LQDDLDDYGF EDYGTDCDNM RVTAFLDIPG QDNLPPLTRL EKYAFSENTF NRQIIARGLL DIFRDFGNNE EDFLTVMEIV VRLSEDAEPT VRTELMEQIP PIAIFLQENR SNFPVVLSEY LIPIVVRYLT DPNNQIICKM ASMLSKSTVE RLLLPRFCEL CGDRKLFQVR KVCAANFGDI CHAVGQEATE KFLIPKFFEL CSDAVWGMRK ACAECFTAVS HSSSPGVRRT QLFPLFIRLV SDPCRWVHQA AFQSLGPFTS TFANPSRAGL YLREDGALSI WPLTQDLDSG FASGSPAPSS GGNTSPASLT RSAKPVRSEP ELPVEGTSAK TSDCPHSSSS SDGPAESPVE SCVSAGAEWT RVSPETSACS KLSDMNDLPI SSYPGSDSWA CPGNTEDVFS HFLYW |
| 分子量 | 45.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人PPP4R1L(或PPP4R2L)蛋白相关的3篇文献摘要示例。*注：PPP4R1L在不同文献中可能被称为PPP4R2L，具体需确认基因命名版本。*
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1. **文献名称**: **"Structural basis of protein phosphatase 4 regulatory subunit function in DNA repair"**
**作者**: Heriche, J.K. et al. (2017)
**摘要**: 揭示了PPP4R1L作为蛋白磷酸酶PP4复合物的调节亚基，通过调控PP4与DNA损伤修复蛋白(如γH2AX)的结合，促进同源重组修复的分子机制。
2. **文献名称**: **"PP4R1/KIAA1622 forms a novel isoform of Protein Phosphatase 4 with regulatory subunit PP4R2"**
**作者**: Zhou, Z. et al. (2008)
**摘要**: 鉴定了PPP4R1L(文中称PP4R2)在PP4复合体中的特异性作用，证明其通过调控PP4磷酸酶活性参与细胞周期检查点及有丝分裂纺锤体组装。
3. **文献名称**: **"Overexpression of PPP4R2 predicts poor prognosis in hepatocellular carcinoma by promoting EMT"**
**作者**: Kang, X. et al. (2019)
**摘要**: 研究指出PPP4R1L(文中用PPP4R2)在肝细胞癌中过表达，通过激活EMT(上皮间质转化)通路促进肿瘤侵袭转移，与患者不良预后显著相关。
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**注意**：以上文献为模拟简化示例，实际研究中请通过PubMed或Google Scholar以**"PPP4R1L"**/**"PPP4R2"**及关键词(如PP4 complex, DNA repair, cancer)检索确认具体文献。
Recombinant human PPP4R1L (protein phosphatase 4 regulatory subunit 1-like) is a protein involved in regulating serine/threonine phosphatase activity. As a regulatory subunit of protein phosphatase 4 (PP4), it forms a complex with the catalytic subunit PPP4C to modulate substrate specificity and phosphatase function. PPP4R1L, also known as SMEK1. shares homology with SMEK2 (PPP4R2) and plays roles in DNA damage response, cell cycle progression, and signal transduction pathways. Its HEAT repeat domains mediate interactions with other proteins, influencing diverse cellular processes.
Research highlights its importance in genome stability maintenance, apoptosis regulation, and immune responses. Dysregulation of PPP4R1L has been implicated in cancers, neurodegenerative disorders, and autoimmune diseases. Recombinant PPP4R1L is typically produced via heterologous expression systems (e.g., E. coli or mammalian cells) for structural and functional studies. Ongoing investigations focus on its therapeutic potential, particularly its interplay with PP4 in dephosphorylating targets like γH2AX and tau proteins. Understanding PPP4R1L's molecular mechanisms could advance treatments for conditions linked to phosphatase dysfunction.
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