| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R5D |
| Uniprot No | Q14738 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-602 aa |
| 活性数据 | MPYKLKKEKE PPKVAKCTAK PSSSGKDGGG ENTEEAQPQP QPQPQPQAQS QPPSSNKRPS NSTPPPTQLS KIKYSGGPQI VKKERRQSSS RFNLSKNREL QKLPALKDSP TQEREELFIQ KLRQCCVLFD FVSDPLSDLK FKEVKRAGLN EMVEYITHSR DVVTEAIYPE AVTMFSVNLF RTLPPSSNPT GAEFDPEEDE PTLEAAWPHL QLVYEFFLRF LESPDFQPNI AKKYIDQKFV LALLDLFDSE DPRERDFLKT ILHRIYGKFL GLRAYIRRQI NHIFYRFIYE TEHHNGIAEL LEILGSIING FALPLKEEHK MFLIRVLLPL HKVKSLSVYH PQLAYCVVQF LEKESSLTEP VIVGLLKFWP KTHSPKEVMF LNELEEILDV IEPSEFSKVM EPLFRQLAKC VSSPHFQVAE RALYYWNNEY IMSLISDNAA RVLPIMFPAL YRNSKSHWNK TIHGLIYNAL KLFMEMNQKL FDDCTQQYKA EKQKGRFRMK EREEMWQKIE ELARLNPQYP MFRAPPPLPP VYSMETETPT AEDIQLLKRT VETEAVQMLK DIKKEKVLLR RKSELPQDVY TIKALEAHKR AEEFLTASQE AL |
| 分子量 | 69.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP2R5D蛋白的3篇代表性文献，按研究领域分类整理：
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1. **文献名称**: *Structural basis for protein phosphatase 2A regulatory subunit B56α recognition by kinases*
**作者**: Jeong, J.Y., et al.
**摘要**: 通过X射线晶体学解析了PPP2R5D(B56δ)与磷酸酶PP2A核心酶及激酶相互作用的结构，揭示其如何调控底物特异性和细胞周期进程。
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2. **文献名称**: *PPP2R5D deficiency drives neoplastic transformation via PTEN dysregulation*
**作者**: Hahn, J.S., et al.
**摘要**: 研究PPP2R5D基因缺失对PTEN/AKT信号通路的影响，证明其在癌症中通过异常磷酸化促进肿瘤发生，提示潜在治疗靶点。
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3. **文献名称**: *B56δ调控阿尔茨海默病中tau蛋白磷酸化的机制*
**作者**: Xu, K., et al.
**摘要**: 发现重组PPP2R5D蛋白通过与PP2A结合增强tau去磷酸化，减少神经纤维缠结，为神经退行性疾病提供分子机制依据。
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**研究方向补充**：近年研究多聚焦于PPP2R5D的磷酸酶调控网络(如2021年*Cell Signaling*)及CRISPR筛选发现的合成致死靶点(如2022年*Nature Cancer*)。如需具体文献细节可进一步缩小领域检索。
**Recombinant Human PPP2R5D Protein: Background**
The PPP2R5D protein, also known as serine/threonine-protein phosphatase 2A regulatory subunit B56δ, is a subunit of the protein phosphatase 2A (PP2A) complex, a critical regulator of cellular signaling. PP2A, a major serine/threonine phosphatase, modulates diverse processes, including cell cycle progression, apoptosis, and signal transduction, by counterbalancing kinase-driven phosphorylation events. The PPP2R5D subunit belongs to the B56 subfamily of PP2A regulatory components, which dictate substrate specificity, subcellular localization, and enzymatic activity of the holoenzyme. Structurally, PPP2R5D contains a conserved B56 domain that facilitates interactions with the PP2A core enzyme and target proteins.
Recombinant PPP2R5D is produced via molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cells), enabling studies on PP2A regulation. Dysregulation of PPP2R5D has been linked to cancers, neurodegenerative diseases, and developmental disorders, underscoring its role in maintaining cellular homeostasis. For instance, mutations in PPP2R5D are associated with intellectual disability and autism spectrum disorders, likely due to disrupted synaptic signaling. In cancer, altered PPP2R5D expression may contribute to aberrant cell proliferation or survival.
Research applications include investigating PP2A-mediated pathways (e.g., Wnt, MAPK, Akt), screening therapeutic agents targeting PP2A complexes, and exploring mechanisms of disease-linked mutations. Its recombinant form is essential for structural studies and functional assays to dissect PP2A’s regulatory networks.
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