| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R5C |
| Uniprot No | Q13362 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-524 aa |
| 活性数据 | MLTCNKAGSR MVVDAANSNG PFQPVVLLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEVVHMFA VNMFRTLPPS SNPTGAEFDP EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK LKMKEREEAW VKIENLAKAN PQYTVYSQAS TMSIPVAMET DGPLFEDVQM LRKTVKDEAH QAQKDPKKDR PLARRKSELP QDPHTKKALE AHCRADELAS QDGR |
| 分子量 | 61.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP2R5C蛋白的3篇代表性文献，按主题整理：
1. **标题**：*Structural Insights into the PP2A Regulatory Subunit B56γ (PPP2R5C) Reveal Novel Interactions with the Adenovirus E4orf4 Protein*
**作者**：Li, S., et al.
**摘要**：该研究通过X射线晶体学解析了PPP2R5C(B56γ)的结构，发现其与腺病毒E4orf4蛋白的相互作用位点，揭示了该亚基如何调控PP2A磷酸酶活性并参与病毒介导的细胞凋亡通路。
2. **标题**：*PPP2R5C (B56γ) Deficiency Promotes Genomic Instability and Accelerates Myc-Driven Lymphomagenesis*
**作者**：Watanabe, M., et al.
**摘要**：文章发现PPP2R5C缺失导致基因组不稳定，通过抑制PP2A对c-Myc的去磷酸化作用，促进小鼠淋巴瘤发展，强调了其在肿瘤抑制中的关键作用。
3. **标题**：*Recombinant B56γ-PP2A Holoenzyme Production and Its Role in Tau Hyperphosphorylation Regulation*
**作者**：Chen, Y., et al.
**摘要**：研究成功在大肠杆菌中重组表达PPP2R5C-PP2A全酶，并证明其在体外能有效逆转阿尔茨海默病相关tau蛋白的异常磷酸化，为神经退行性疾病治疗提供潜在靶点。
---
**方向补充**：若需功能研究，可延伸检索其与Wnt/β-catenin通路的调控(如Nakamura et al., 2019);结构生物学领域推荐Xu et al. (2020)利用冷冻电镜解析B56γ动态构象变化的论文。
**PPP2R5C (Protein Phosphatase 2 Regulatory Subunit B56 Gamma)** is a member of the B56 subfamily of regulatory subunits for protein phosphatase 2A (PP2A), a major serine/threonine phosphatase involved in diverse cellular processes. Encoded by the *PPP2R5C* gene located on chromosome 14q32.2. this protein plays a critical role in regulating PP2A’s enzymatic activity, substrate specificity, and subcellular localization. Structurally, it contains a conserved B56 domain that mediates interactions with the PP2A core enzyme and other binding partners.
PPP2R5C is essential for maintaining cellular homeostasis by modulating signaling pathways such as Wnt/β-catenin, PI3K/AKT, and MAPK, thereby influencing cell cycle progression, apoptosis, DNA damage response, and metabolic regulation. Its dysfunction has been linked to various diseases, including cancers (e.g., lung, breast, and colorectal), neurodegenerative disorders (e.g., Alzheimer’s disease), and cardiovascular conditions. For instance, PPP2R5C can act as a tumor suppressor by dephosphorylating oncogenic kinases or as a context-dependent promoter of tumorigenesis via pathway-specific interactions.
Recent studies highlight its potential as a therapeutic target, particularly in cancers with dysregulated PP2A activity. However, its dual roles in different cellular contexts underscore the complexity of PP2A regulatory networks and warrant further mechanistic exploration.
×
