| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R5B |
| Uniprot No | Q15173 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-497 aa |
| 活性数据 | METKLPPAST PTSPSSPGLS PVPPPDKVDG FSRRSLRRAR PRRSHSSSQF RYQSNQQELT PLPLLKDVPA SELHELLSRK LAQCGVMFDF LDCVADLKGK EVKRAALNEL VECVGSTRGV LIEPVYPDII RMISVNIFRT LPPSENPEFD PEEDEPNLEP SWPHLQLVYE FFLRFLESPD FQPSVAKRYV DQKFVLMLLE LFDSEDPRER EYLKTILHRV YGKFLGLRAY IRKQCNHIFL RFIYEFEHFN GVAELLEILG SIINGFALPL KTEHKQFLVR VLIPLHSVKS LSVFHAQLAY CVVQFLEKDA TLTEHVIRGL LKYWPKTCTQ KEVMFLGEME EILDVIEPSQ FVKIQEPLFK QVARCVSSPH FQVAERALYF WNNEYILSLI EDNCHTVLPA VFGTLYQVSK EHWNQTIVSL IYNVLKTFME MNGKLFDELT ASYKLEKQQE QQKAQERQEL WQGLEELRLR RLQGTQGAKE APLQRLTPQV AASGGQS |
| 分子量 | 57.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP2R5B蛋白的模拟参考文献示例(基于领域内常见研究方向，非真实文献)：
1. **“Structural and Functional Characterization of Recombinant Human PPP2R5B in PP2A Complex Assembly”**
*作者：Smith J. et al. (2018)*
摘要：本研究通过大肠杆菌系统表达并纯化了重组PPP2R5B蛋白，揭示了其与PP2A核心酶的相互作用机制，证明PPP2R5B通过调节底物特异性影响PP2A的磷酸酶活性，为靶向PP2A复合物的药物设计提供依据。
2. **“PPP2R5B Modulates Wnt/β-Catenin Signaling via Regulation of β-Catenin Phosphorylation”**
*作者：Zhang L. et al. (2020)*
摘要：利用重组PPP2R5B蛋白进行的体外实验表明，其通过调控β-catenin的磷酸化状态影响Wnt信号通路，在细胞增殖和分化中起关键作用，提示PPP2R5B可能作为癌症治疗的潜在靶点。
3. **“Cryo-EM Structure of PPP2R5B Reveals Mechanisms of Cancer-Associated Mutations”**
*作者：Johnson R. et al. (2021)*
摘要：通过冷冻电镜解析重组PPP2R5B的高分辨率结构，发现肿瘤中高频突变位点破坏其与PP2A的结合，导致信号通路异常，阐明了PPP2R5B突变在癌症发生中的分子机制。
4. **“Recombinant PPP2R5B Expression in Mammalian Cells and Role in Apoptosis Regulation”**
*作者：Chen X. et al. (2019)*
摘要：在HEK293细胞中表达重组PPP2R5B，发现其通过PI3K/AKT通路调控细胞凋亡，为神经退行性疾病与PPP2R5B功能异常的关联提供了实验证据。
---
**注**：以上为模拟文献，实际文献建议通过PubMed或Google Scholar以“PPP2R5B”、“recombinant PPP2R5B”等关键词检索获取。
**Background of Recombinant Human PPP2R5B Protein**
PPP2R5B (Protein Phosphatase 2 Regulatory Subunit B' Beta), also known as B56β, is a regulatory subunit of the serine/threonine protein phosphatase 2A (PP2A) complex. PP2A is a major cellular phosphatase involved in diverse processes, including cell cycle regulation, apoptosis, and signal transduction. The PPP2R5B subunit belongs to the B56 family of regulatory subunits, which dictate substrate specificity, subcellular localization, and enzymatic activity of the PP2A holoenzyme. Structurally, PPP2R5B contains seven WD40 repeats that facilitate protein-protein interactions and mediate binding to specific substrates or scaffolding proteins.
Recombinant PPP2R5B protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) for biochemical and functional studies. It aids in dissecting PP2A's role in signaling pathways, such as the AKT, MAPK, and Wnt pathways, and its interactions with viral proteins or oncogenic targets. Dysregulation of PPP2R5B has been implicated in cancers, neurodegenerative diseases, and metabolic disorders, highlighting its therapeutic potential. Studies using recombinant PPP2R5B have also advanced structural biology efforts, revealing mechanisms of PP2A assembly and regulation. Its applications extend to drug discovery, particularly in targeting PP2A-associated pathologies.
×
