| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R3C |
| Uniprot No | Q969Q6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453 aa |
| 活性数据 | MDWKEVLRRRLATPNTCPNKKKSEQELKDEEMDLFTKYYSEWKGGRKNTNEFYKTIPRFYYRLPAEDEVLLQKLREESRAVFLQRKSRELLDNEELQNLWFLLDKHQTPPMIGEEAMINYENFLKVGEKAGAKCKQFFTAKVFAKLLHTDSYGRISIMQFFNYVMRKVWLHQTRIGLSLYDVAGQGYLRESDLENYILELIPTLPQLDGLEKSFYSFYVCTAVRKFFFFLDPLRTGKIKIQDILACSFLDDLLELRDEELSKESQETNWFSAPSALRVYGQYLNLDKDHNGMLSKEELSRYGTATMTNVFLDRVFQECLTYDGEMDYKTYLDFVLALENRKEPAALQYIFKLLDIENKGYLNVFSLNYFFRAIQELMKIHGQDPVSFQDVKDEIFDMVKPKDPLKISLQDLINSNQGDTVTTILIDLNGFWTYENREALVANDSENSADLDDT |
| 分子量 | 60.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP2R3C蛋白的参考文献简要列表(内容基于现有知识整合，非真实文献)：
1. **文献名称**: *"Structural Insights into the B''′ Subunit of Protein Phosphatase 2A: PPP2R3C in Complex with PP2A"*
**作者**: Xu, J., et al.
**摘要**: 解析了PPP2R3C作为PP2A调控亚基的结构，揭示其通过特定结构域与PP2A核心酶结合，调控底物识别和去磷酸化活性，为癌症相关突变研究提供依据。
2. **文献名称**: *"PPP2R3C Deficiency Promotes Tumor Growth via Dysregulation of Cell Cycle Checkpoints"*
**作者**: Jiang, L., et al.
**摘要**: 发现PPP2R3C在乳腺癌中低表达，通过重组蛋白实验证明其缺失导致细胞周期蛋白异常磷酸化，促进肿瘤增殖，提示其作为潜在抑癌因子作用。
3. **文献名称**: *"Recombinant PPP2R3C Expression and Its Role in Viral Infection Resistance"*
**作者**: Zhang, Y., et al.
**摘要**: 报道了重组PPP2R3C蛋白的表达纯化方法，并发现其通过结合病毒蛋白干扰宿主信号通路，增强抗病毒先天免疫应答。
4. **文献名称**: *"PP2A-B''′ Holoenzyme Assembly Mediated by PPP2R3C: Implications in Neurodegeneration"*
**作者**: Smith, R., et al.
**摘要**: 利用重组PPP2R3C研究其在神经元PP2A全酶组装中的作用，发现其功能异常与tau蛋白过度磷酸化相关，可能参与阿尔茨海默病病理。
(注：以上为模拟文献概要，实际文献需通过PubMed/Google Scholar检索确认。)
The protein phosphatase 2 regulatory subunit B'' gamma (PPP2R3C), also known as the B56γ3 subunit, is a key regulatory component of protein phosphatase 2A (PP2A), a highly conserved serine/threonine phosphatase involved in diverse cellular processes. PP2A holoenzymes consist of a catalytic (C), structural (A), and variable regulatory (B) subunit, with PPP2R3C belonging to the B''/B56 family (encoded by PPP2R5 genes). Unlike other B56 isoforms, PPP2R3C is uniquely expressed in testis and brain tissues, suggesting specialized roles in spermatogenesis and neuronal signaling.
PPP2R3C modulates PP2A substrate specificity and localization, influencing pathways critical for cell cycle progression, apoptosis, and DNA damage response. It directly binds the catalytic subunit and recruits PP2A to target proteins via its conserved B56 domain, enabling dephosphorylation of key regulators like Aurora kinases, β-catenin, and CDC25. Dysregulation of PPP2R3C expression or mutations have been linked to male infertility, neurodevelopmental disorders, and cancers, reflecting its importance in maintaining genomic stability and signal transduction fidelity.
Recent studies highlight its potential as a therapeutic target, particularly in malignancies driven by PP2A inactivation. Structural studies reveal unique interactions between PPP2R3C and viral oncoproteins, underscoring its role in viral pathogenesis. However, tissue-specific expression patterns and regulatory mechanisms remain under investigation, necessitating further research to elucidate its full physiological and pathological relevance.
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