| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R2D |
| Uniprot No | Q66LE6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453 aa |
| 活性数据 | MAGAGGGGCP AGGNDFQWCF SQVKGAIDED VAEADIISTV EFNYSGDLLA TGDKGGRVVI FQREQENKSR PHSRGEYNVY STFQSHEPEF DYLKSLEIEE KINKIRWLPQ QNAAHFLLST NDKTIKLWKI SERDKRAEGY NLKDEDGRLR DPFRITALRV PILKPMDLMV EASPRRIFAN AHTYHINSIS VNSDHETYLS ADDLRINLWH LEITDRSFNI VDIKPANMEE LTEVITAAEF HPHQCNVFVY SSSKGTIRLC DMRSSALCDR HSKFFEEPED PSSRSFFSEI ISSISDVKFS HSGRYMMTRD YLSVKVWDLN MESRPVETHQ VHEYLRSKLC SLYENDCIFD KFECCWNGSD SAIMTGSYNN FFRMFDRDTR RDVTLEASRE SSKPRASLKP RKVCTGGKRR KDEISVDSLD FNKKILHTAW HPVDNVIAVA ATNNLYIFQD KIN |
| 分子量 | 52.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人PPP2R2D蛋白的示例参考文献(内容为虚构，仅供格式参考)：
1. **标题**：PPP2R2D structure and phosphatase regulation in cancer cells
**作者**：Chen et al. (2020)
**摘要**：解析了PPP2R2D蛋白通过其B56结构域与PP2A催化亚基结合的分子机制，发现其重组蛋白在肝癌细胞中通过调控MAPK通路抑制增殖。
2. **标题**：Recombinant PPP2R2D expression alters neuronal differentiation via AKT signaling
**作者**：Li & Wang (2018)
**摘要**：利用HEK293细胞表达重组PPP2R2D，证实其通过调控PP2A-AKT相互作用影响神经元分化，为神经发育疾病提供潜在机制。
3. **标题**：PPP2R2D genetic variants link to autoimmune disease susceptibility
**作者**：Rodríguez et al. (2021)
**摘要**：通过全基因组关联分析，发现PPP2R2D基因突变与系统性红斑狼疮相关，重组蛋白实验显示突变体影响T细胞受体的去磷酸化过程。
**提示**：以上文献为模拟内容，实际研究请通过PubMed/Google Scholar检索关键词：PPP2R2D、recombinant protein、PP2A regulatory subunit等，并筛选近五年高被引论文。
Recombinant human PPP2R2D (Protein Phosphatase 2 Regulatory Subunit Bdelta) is a key regulatory component of the protein phosphatase 2 (PP2A) complex, a Ser/Thr phosphatase critical for cellular signaling and homeostasis. As a member of the B55 regulatory subfamily, PPP2R2D directs PP2A’s substrate specificity, enzymatic activity, and subcellular localization by forming heterotrimeric holoenzymes with a catalytic (C) and scaffold (A) subunit. It plays a role in diverse processes, including cell cycle regulation, apoptosis, DNA damage response, and neuronal signaling, often acting as a tumor suppressor by counterbalancing kinase-driven signaling pathways.
The recombinant form of PPP2R2D is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure-function relationships, interaction networks, and disease associations. Mutations or dysregulation of PPP2R2D have been implicated in cancers, neurodegenerative disorders, and developmental defects, underscoring its therapeutic relevance. Research using recombinant PPP2R2D aids in deciphering PP2A's regulatory mechanisms and developing targeted therapies to restore phosphatase activity in pathological conditions. Its study also provides insights into isoform-specific roles within the B55 family, advancing precision medicine approaches.
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