| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R2A |
| Uniprot No | P63151 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-447 aa |
| 活性数据 | AGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQENKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKRPEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRINLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFEEPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYENDCIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISVDSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN |
| 分子量 | 58.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP2R2A蛋白的参考文献示例(部分内容为假设性概括，仅供参考)：
1. **"Structural insights into PP2A regulation by PPP2R2A through cryo-EM analysis"**
- 作者：Zhang Y et al.
- 摘要：该研究通过冷冻电镜解析了PPP2R2A与PP2A核心酶复合物的三维结构，揭示了PPP2R2A通过其B亚基特异性介导底物识别和定位的分子机制。
2. **"PPP2R2A downregulation promotes tumorigenesis in colorectal cancer via Wnt/β-catenin pathway activation"**
- 作者：Chen L et al.
- 摘要：研究发现PPP2R2A在结直肠癌中表达下调，其缺失导致PP2A磷酸酶活性降低，进而激活Wnt/β-catenin信号通路，促进肿瘤增殖和转移。
3. **"Recombinant expression and functional characterization of human PPP2R2A in neuronal differentiation"**
- 作者：Kim S et al.
- 摘要：通过重组表达PPP2R2A蛋白，验证其在神经元分化中调控MAPK信号通路的作用，表明该蛋白通过去磷酸化关键激酶影响神经细胞命运。
4. **"PP2A-B55α holoenzyme regulation and cancer-specific mutations in PPP2R2A"**
- 作者：Hahn A et al.
- 摘要：分析了PPP2R2A基因在癌症中的突变热点，并通过体外重组蛋白实验证明突变导致PP2A酶活异常，可能与肿瘤耐药性相关。
注：以上文献为示例，实际引用需核对真实发表的论文及摘要内容。建议通过PubMed、Web of Science或Google Scholar搜索关键词“PPP2R2A recombinant”或“PPP2R2A function”获取最新文献。
The protein phosphatase 2 regulatory subunit B alpha (PPP2R2A) is a key component of the protein phosphatase 2A (PP2A) complex, a major serine/threonine phosphatase involved in regulating diverse cellular processes. PP2A functions as a heterotrimeric enzyme composed of a structural (A), catalytic (C), and regulatory (B) subunit. PPP2R2A belongs to the B55/B subfamily of regulatory subunits, which determines substrate specificity, subcellular localization, and enzymatic activity of the holoenzyme. It plays critical roles in cell cycle regulation, apoptosis, DNA damage response, and signal transduction pathways (e.g., Wnt, MAPK, and AKT), often acting as a tumor suppressor. Dysregulation of PPP2R2A has been linked to cancers, neurodegenerative disorders, and cardiovascular diseases. Recombinant human PPP2R2A protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies on PP2A assembly, substrate recognition, and its interaction with small molecules or pathogenic proteins. Its application spans structural biology, enzymology, and drug discovery, particularly in targeting PP2A-related pathways for therapeutic intervention. Researchers utilize tagged versions (e.g., His-tag) for purification and interaction assays, aiding in elucidating mechanisms underlying PP2A’s regulatory roles in health and disease.
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