| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R21 |
| Uniprot No | Q6ZMI0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-780 aa |
| 活性数据 | MASAELQGKY QKLAQEYSKL RAQNQVLKKG VVDEQANSAA LKEQLKMKDQ SLRKLQQEMD SLTFRNLQLA KRVELLQDEL ALSEPRGKKN KKSGESSSQL SQEQKSVFDE DLQKKIEENE RLHIQFFEAD EQHKHVEAEL RSRLATLETE AAQHQAVVDG LTRKYMETIE KLQNDKAKLE VKSQTLEKEA KECRLRTEEC QLQLKTLHED LSGRLEESLS IINEKVPFND TKYSQYNALN VPLHNRRHQL KMRDIAGQAL AFVQDLVTAL LNFHTYTEQR IQIFPVDSAI DTISPLNQKF SQYLHENASY VRPLEEGMLH LFESITEDTV TVLETTVKLK TFSEHLTSYI CFLRKILPYQ LKSLEEECES SLCTSALRAR NLELSQDMKK MTAVFEKLQT YIALLALPST EPDGLLRTNY SSVLTNVGAA LHGFHDVMKD ISKHYSQKAA IEHELPTATQ KLITTNDCIL SSVVALTNGA GKIASFFSNN LDYFIASLSY GPKAASGFIS PLSAECMLQY KKKAAAYMKS LRKPLLESVP YEEALANRRI LLSSTESREG LAQQVQQSLE KISKLEQEKE HWMLEAQLAK IKLEKENQRI ADKLKNTGSA QLVGLAQENA AVSNTAGQDE ATAKAVLEPI QSTSLIGTLT RTSDSEVPDV ESREDLIKNH YMARIVELTS QLQLADSKSV HFYAECRALS KRLALAEKSK EALTEEMKLA SQNISRLQDE LTTTKRSYED QLSMMSDHLC SMNETLSKQR EEIDTLKMSS KGNSKKNKSR |
| 分子量 | 88.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP1R21蛋白的3篇代表性参考文献示例(注：部分内容为模拟虚构，供参考)：
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1. **文献名称**：*PPP1R21 regulates ciliogenesis through interaction with PDE6D in human cells*
**作者**：Chen L et al.
**摘要**：本研究揭示了PPP1R21在纤毛形成中的作用，发现其通过结合磷酸二酯酶PDE6D调控纤毛基底体的运输。利用重组PPP1R21蛋白进行体外互作实验，证实其与PDE6D的直接结合，并表明该互作缺陷与纤毛病相关。
2. **文献名称**：*Structural insights into the binding mechanism of PPP1R21 with protein phosphatase 1*
**作者**：Wang Y et al.
**摘要**：通过X射线晶体学解析重组人PPP1R21蛋白与PP1的复合物结构，阐明了PPP1R21中保守的RVXF基序如何锚定PP1催化亚基，并调节其底物特异性，为靶向PP1调控网络提供分子基础。
3. **文献名称**：*PPP1R21 deficiency disrupts neuronal development via mTOR signaling dysregulation*
**作者**：Gomez-Sanchez JA et al.
**摘要**：在小鼠模型和人源重组PPP1R21蛋白功能实验中，发现PPP1R21缺失导致mTOR通路过度激活，引发神经元迁移障碍和自噬异常，提示其在神经发育疾病中的潜在作用。
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如需真实文献，建议通过PubMed或Google Scholar以“PPP1R21 recombinant”等关键词检索。
**Background of Recombinant Human PPP1R21 Protein**
Recombinant human PPP1R21 (Protein Phosphatase 1 Regulatory Subunit 21) is a regulatory protein that interacts with protein phosphatase 1 (PP1), a critical serine/threonine phosphatase involved in diverse cellular processes. PPP1R21 contains conserved PP1-binding motifs (e.g., RVxF and SILK), enabling it to modulate PP1’s substrate specificity and activity. Structurally, PPP1R21 includes a C-terminal domain with transmembrane regions, suggesting potential roles in membrane-associated signaling or organelle regulation.
Functionally, PPP1R21 is implicated in intracellular trafficking, lysosomal homeostasis, and Golgi apparatus organization. Studies link PPP1R21 mutations to developmental disorders, including intellectual disability and craniofacial anomalies, underscoring its importance in neurodevelopment. Its recombinant form, produced via bacterial or mammalian expression systems, retains binding affinity for PP1 and is utilized to study molecular mechanisms, protein-protein interactions, and disease-associated pathways.
Current research explores PPP1R21’s role in autophagy and endosomal sorting, potentially intersecting with mTOR signaling. As a tool, recombinant PPP1R21 aids in deciphering PP1’s regulatory networks and validating therapeutic targets for neurological or lysosomal disorders. Further investigations aim to clarify its structural dynamics and disease-specific regulatory cascades.
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