| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R14C |
| Uniprot No | Q8TAE6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-165 aa |
| 活性数据 | SVATGSSET AGGASGGGAR VFFQSPRGGA GGSPGSSSGS GSSREDSAPV ATAAAAGQVQ QQQQRRHQQG KVTVKYDRKE LRKRLVLEEW IVEQLGQLYG CEEEEMPEVE IDIDDLLDAD SDEERASKLQ EALVDCYKPT EEFIKELLSR IRGMRKLSPP QKKSV |
| 分子量 | 17.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP1R14C蛋白的3篇参考文献示例(注：文献信息为示例性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**：PPP1R14C regulates lung cancer progression via ERK signaling pathway
**作者**：Zhijie Li et al. (2018)
**摘要**：研究发现PPP1R14C通过抑制蛋白磷酸酶1(PP1)活性，激活ERK信号通路，促进非小细胞肺癌细胞的增殖和迁移。
2. **文献名称**：Role of PPP1R14C in vascular smooth muscle contraction
**作者**：Hiroshi Shimizu et al. (2015)
**摘要**：PPP1R14C通过调控肌球蛋白轻链磷酸化水平，影响血管平滑肌收缩功能，为高血压病理机制提供了新视角。
3. **文献名称**：PPP1R14C modulates apoptosis under oxidative stress
**作者**：Samantha K. Smith et al. (2020)
**摘要**：在氧化应激条件下，PPP1R14C与JNK通路相互作用，促进线粒体依赖性细胞凋亡，提示其在神经退行性疾病中的潜在作用。
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建议通过PubMed或Web of Science检索关键词“PPP1R14C”或“Protein Phosphatase 1 Regulatory Subunit 14C”获取真实文献。
**Background of Recombinant Human PPP1R14C Protein**
PPP1R14C (Protein Phosphatase 1 Regulatory Subunit 14C), also known as CPI-17. is a key regulatory protein involved in cellular signaling pathways. It belongs to a family of phosphatase inhibitor proteins that modulate the activity of protein phosphatase 1 (PP1), a critical enzyme responsible for dephosphorylating serine/threonine residues in target proteins. PPP1R14C specifically binds to PP1. inhibiting its enzymatic activity and thereby influencing the phosphorylation status of downstream effectors. This regulation is vital for processes such as smooth muscle contraction, neuronal signaling, and cellular proliferation.
Structurally, PPP1R14C contains a conserved central PP1-binding motif and a C-terminal region critical for its inhibitory function. Its activity is tightly regulated by phosphorylation, particularly at Thr-38. which is mediated by kinases like protein kinase C (PKC) or Rho-associated kinase (ROCK). Upon phosphorylation, PPP1R14C undergoes conformational changes, enhancing its affinity for PP1 and amplifying its inhibitory effects.
Dysregulation of PPP1R14C has been implicated in pathologies, including hypertension, cancer, and neurological disorders. For instance, overexpression in certain cancers correlates with increased cell survival and chemoresistance. Recombinant PPP1R14C protein, typically produced in *E. coli* or mammalian expression systems, serves as a tool to study PP1-mediated signaling mechanisms and screen therapeutic agents targeting PP1-associated pathways. Its role in smooth muscle tone regulation also makes it a potential candidate for drug development in cardiovascular diseases.
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