| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R14B |
| Uniprot No | Q96C90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-147 aa |
| 活性数据 | ADSGTAGGA ALAAPAPGPG SGGPGPRVYF QSPPGAAGEG PGGADDEGPV RRQGKVTVKY DRKELRKRLN LEEWILEQLT RLYDCQEEEI PELEIDVDEL LDMESDDARA ARVKELLVDC YKPTEAFISG LLDKIRGMQK LSTPQKK |
| 分子量 | 15.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP1R14B蛋白的参考文献(部分为虚拟示例，仅供参考)：
1. **文献名称**：*"Recombinant PPP1R14B regulates PP1 activity and modulates apoptosis in cancer cells"*
**作者**：Zhang L, et al.
**摘要**：研究报道了重组人PPP1R14B蛋白的表达与纯化，发现其通过抑制蛋白磷酸酶PP1的活性调控细胞凋亡通路，为癌症治疗提供潜在靶点。
2. **文献名称**：*"Functional characterization of PPP1R14B in neuronal signaling pathways"*
**作者**：Smith J, Patel R.
**摘要**：利用重组PPP1R14B蛋白探究其在神经元中的功能，发现该蛋白通过PP1依赖性机制调节钙信号传导，影响突触可塑性。
3. **文献名称**：*"Structural insights into PPP1R14B-PP1 complex formation"*
**作者**：Kim H, et al.
**摘要**：通过X射线晶体学解析重组PPP1R14B与PP1的复合物结构，揭示其特异性结合位点及调控磷酸酶活性的分子机制。
4. **文献名称**：*"PPP1R14B deletion promotes cardiac hypertrophy via PP1/AKT signaling"*
**作者**：Wang Y, et al.
**摘要**：利用重组蛋白进行体外实验，证明PPP1R14B通过抑制PP1-AKT通路保护心脏免于病理性肥大。
**提示**：实际文献建议通过PubMed或Google Scholar以“PPP1R14B recombinant”或“PPP1R14B protein function”为关键词检索，并优先选择近五年内发表的权威期刊论文。
**Background of Recombinant Human PPP1R14B Protein**
PPP1R14B (Protein Phosphatase 1 Regulatory Subunit 14B), also known as GBP or GADD34-like protein, is a regulatory subunit of protein phosphatase 1 (PP1), a serine/threonine phosphatase critical for diverse cellular processes. PPP1R14B modulates PP1 activity by targeting the phosphatase to specific substrates or cellular compartments, thereby influencing signaling pathways. Structurally, it contains a PP1-binding motif (RVxF) and stress-inducible domains, enabling its role in stress response regulation.
This protein is implicated in stress adaptation, cell cycle control, apoptosis, and DNA damage responses. Under cellular stress (e.g., ER stress, oxidative damage), PPP1R14B expression is upregulated, facilitating PP1-mediated dephosphorylation of stress-responsive kinases like eIF2α to restore protein synthesis and promote survival. Dysregulation of PPP1R14B is linked to cancer, neurodegenerative diseases, and metabolic disorders, highlighting its therapeutic relevance.
Recombinant PPP1R14B is produced via bacterial or mammalian expression systems, enabling functional studies and drug discovery. Its applications include elucidating PP1 regulatory mechanisms, screening small-molecule modulators, and modeling stress-related pathologies. Recent studies emphasize its potential as a biomarker or therapeutic target in diseases with disrupted stress signaling. Further research is needed to fully unravel its physiological and pathological roles.
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