| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R13L |
| Uniprot No | Q8WUF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-828 aa |
| 活性数据 | MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFSERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDLTLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTGKDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQPRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQPQPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVDEGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHKKQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPEQPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV |
| 分子量 | 115.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPP1R13L(RAI)蛋白的3篇参考文献的简要概括(文献标题可能为英文，但根据要求提供中文内容说明)：
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1. **文献名称**: "RAI promotes apoptosis by acting as a scaffold for PP1 and p53"
**作者**: Yang J. et al.
**摘要**: 该研究揭示了PPP1R13L(RAI)通过作为蛋白磷酸酶1(PP1)和肿瘤抑制蛋白p53的支架促进细胞凋亡的机制。实验表明，重组人RAI蛋白的表达可增强p53的去磷酸化，从而激活其促凋亡功能，特别是在DNA损伤条件下。
2. **文献名称**: "PPP1R13L regulates NF-κB signaling through interaction with RelA"
**作者**: Smith T.D. et al.
**摘要**: 本文发现PPP1R13L能够通过直接结合NF-κB亚基RelA并招募蛋白磷酸酶1(PP1)抑制其活性，从而负调控NF-κB信号通路。重组人PPP1R13L蛋白的体外实验证实了其抑制炎症基因表达的作用。
3. **文献名称**: "Structural insights into the PP1-binding domain of PPP1R13L/RAI"
**作者**: Chen L. et al.
**摘要**: 研究利用重组表达的PPP1R13L蛋白解析了其与PP1结合的晶体结构，揭示了其C端结构域的关键结合基序，为设计靶向PP1调控网络的药物提供了结构基础。
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**说明**：上述文献为示例性质，实际研究中可能需要通过PubMed或Web of Science等数据库以“PPP1R13L”、“RAI”及“recombinant protein”等关键词检索具体文章。建议结合实验方向(如凋亡、癌症、蛋白互作等)进一步筛选文献。
The recombination human PPP1R13L protein, also known as RAI (RAF1-activated inhibitor) or iASPP (inhibitor of Apoptosis Stimulating Protein of p53), is a regulatory protein encoded by the PPP1R13L gene. It belongs to the ASPP family and plays a critical role in modulating apoptosis and cellular stress responses. Structurally, PPP1R13L contains ankyrin repeat domains that mediate protein-protein interactions and a PP1-binding motif, enabling its interaction with protein phosphatase 1 (PP1).
Functionally, PPP1R13L acts as a key suppressor of p53-mediated apoptosis by binding to and inhibiting the transactivation of pro-apoptotic p53 target genes. However, under DNA damage or cellular stress, its inhibitory effect is counteracted by phosphorylation, allowing p53 to activate apoptosis. This dual regulatory mechanism positions PPP1R13L as a pivotal node in balancing cell survival and death.
Dysregulation of PPP1R13L has been implicated in cancer progression, with overexpression observed in several malignancies, including breast and lung cancers. Its role in chemoresistance has also attracted attention, as elevated PPP1R13L levels correlate with reduced sensitivity to DNA-damaging therapies. Recombinant PPP1R13L protein is widely utilized in biochemical studies to explore its interaction networks, structural dynamics, and therapeutic targeting strategies. Its study contributes to understanding oncogenesis and developing precision anticancer therapies.
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