| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R12C |
| Uniprot No | Q9BZL4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-782 aa |
| 活性数据 | SGEDGPAAG PGAAAAAARE RRREQLRQWG ARAGAEPGPG ERRARTVRFE RAAEFLAACA GGDLDEARLM LRAADPGPGA ELDPAAPPPA RAVLDSTNAD GISALHQACI DENLEVVRFL VEQGATVNQA DNEGWTPLHV AASCGYLDIA RYLLSHGANI AAVNSDGDLP LDLAESDAME GLLKAEIARR GVDVEAAKRA EEELLLHDTR CWLNGGAMPE ARHPRTGASA LHVAAAKGYI EVMRLLLQAG YDPELRDGDG WTPLHAAAHW GVEDACRLLA EHGGGMDSLT HAGQRPCDLA DEEVLSLLEE LARKQEDLRN QKEASQSRGQ EPQAPSSSKH RRSSVCRLSS REKISLQDLS KERRPGGAGG PPIQDEDEGE EGPTEPPPAE PRTLNGVSSP PHPSPKSPVQ LEEAPFSRRF GLLKTGSSGA LGPPERRTAE GAPGAGLQRS ASSSWLEGTS TQAKELRLAR ITPTPSPKLP EPSVLSEVTK PPPCLENSSP PSRIPEPESP AKPNVPTAST APPADSRDRR RSYQMPVRDE ESESQRKARS RLMRQSRRST QGVTLTDLKE AEKAAGKAPE SEKPAQSLDP SRRPRVPGVE NSDSPAQRAE APDGQGPGPQ AAREHRKVGK EWRGPAEGEE AEPADRSQES STLEGGPSAR RQRWQRDLNP EPEPESEEPD GGFRTLYAEL RRENERLREA LTETTLRLAQ LKVELERATQ RQERFAERPA LLELERFERR ALERKAAELE EELKALSDLR ADNQRLKDEN AALIRVISKL SK |
| 分子量 | 84.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural insights into the regulation of myosin phosphatase by the alternative splice variant of the regulatory subunit, MYPT3"** by Matsuura et al.
- **摘要**：研究报道了重组人PPP1R12C(MYPT3)蛋白的表达与纯化，通过晶体学分析其与蛋白磷酸酶PP1的相互作用，揭示其对肌球蛋白磷酸酶活性的变构调节机制。
2. **"Recombinant production and functional characterization of the PP1 regulatory subunit PPP1R12C"** by Eto et al.
- **摘要**：利用大肠杆菌重组系统表达PPP1R12C，验证其作为PP1抑制剂的功能，证明其在细胞周期调控中通过去磷酸化特定底物发挥作用。
3. **"Tissue-specific roles of MYPT family members in smooth muscle contractility"** by Grassie et al.
- **摘要**：通过重组蛋白实验比较PPP1R12C(MYPT3)与其他亚型(如MYPT1)的功能差异，发现其在神经和平滑肌中调控钙敏感性途径的独特作用。
4. **"PPP1R12C modulates apoptosis signaling by binding to the Bcl-2 family proteins"** by Liu et al.
- **摘要**：利用重组PPP1R12C蛋白探究其与凋亡相关蛋白的互作，发现其通过结合Bcl-2家族成员调控癌症细胞的存活与死亡。
(注：以上文献标题及作者为虚拟示例，实际研究中请根据具体论文调整。)
**Background of Recombinant Human PPP1R12C Protein**
PPP1R12C (Protein Phosphatase 1 Regulatory Subunit 12C), also known as MYPT3 (Myosin Phosphatase Target Subunit 3), is a regulatory subunit of protein phosphatase 1 (PP1), a critical serine/threonine phosphatase involved in diverse cellular processes. This protein plays a pivotal role in modulating the activity of myosin phosphatase, which regulates smooth muscle contraction, cytoskeletal organization, and cell motility by dephosphorylating myosin light chains.
Structurally, PPP1R12C contains multiple functional domains, including a PP1-binding motif, a central coiled-coil region, and a C-terminal leucine-zipper domain, enabling interactions with PP1 catalytic subunits (e.g., PP1c) and other regulatory partners. It is implicated in signaling pathways such as RhoA/ROCK, which influences cell contractility, migration, and apoptosis. Dysregulation of PPP1R12C has been linked to diseases like hypertension, cancer metastasis, and neurological disorders, highlighting its therapeutic potential.
Recombinant human PPP1R12C protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research. It serves as a vital tool for studying PP1-mediated dephosphorylation mechanisms, drug screening, and elucidating its role in cellular physiology and pathology.
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