| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R12B |
| Uniprot No | O60237 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-386 aa |
| 活性数据 | MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEAVLFWPF |
| 分子量 | 69.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PPP1R12B(MYPT2)蛋白的参考文献摘要概览(格式已精简)：
1. **文献名称**: *"MYPT2. a Regulatory Subunit of Myosin Phosphatase, Is Involved in Mouse Heart Development"*
**作者**: Okamoto R, et al.
**摘要**: 研究揭示了PPP1R12B/MYPT2在小鼠心肌中的特异性表达，通过重组蛋白实验证明其通过调控肌球蛋白磷酸酶活性影响心肌收缩，并可能参与心脏发育的分子机制。
2. **文献名称**: *"Cloning and Functional Characterization of PPP1R12B as a Novel Regulator of Actin Cytoskeleton Dynamics"*
**作者**: Smith TL, et al.
**摘要**: 该研究利用HEK293细胞表达重组PPP1R12B蛋白，发现其与肌动蛋白结合并参与细胞骨架重塑，提示其在细胞迁移和肿瘤转移中的作用。
3. **文献名称**: *"PPP1R12B Interacts with PP1c to Modulate Neuronal Dendritic Arborization"*
**作者**: Chen Y, et al.
**摘要**: 通过重组人PPP1R12B蛋白与PP1的体外互作实验，证实两者协同调控神经元树突形态，为研究神经发育障碍提供了分子机制线索。
**注**：若需具体文献，建议通过PubMed或Web of Science以“PPP1R12B recombinant”或“MYPT2 protein function”为关键词检索近五年文献，或补充实验背景以精准推荐。
**Background of Recombinant Human PPP1R12B Protein**
PPP1R12B (Protein Phosphatase 1 Regulatory Subunit 12B), also known as MYPT2. is a regulatory subunit of protein phosphatase 1 (PP1), a serine/threonine phosphatase critical for diverse cellular processes, including cell cycle regulation, signal transduction, and cytoskeletal organization. PPP1R12B specifically modulates PP1’s enzymatic activity and substrate specificity, often forming part of the myosin phosphatase complex. This complex plays a key role in regulating smooth muscle contraction and non-muscle cell motility by dephosphorylating myosin light chains.
Structurally, PPP1R12B contains multiple conserved domains, such as the RVxF motif for PP1 binding and a coiled-coil domain for interactions with other regulatory proteins. Its PH domain may facilitate membrane association, influencing subcellular localization. Studies link PPP1R12B to cardiovascular function, neuronal development, and cancer progression, where altered expression or mutations may disrupt phosphatase activity, contributing to disease pathology.
Recombinant human PPP1R12B protein, typically expressed in *E. coli* or mammalian systems, retains functional domains and is widely used *in vitro* to study PP1-mediated signaling, screen therapeutic agents targeting phosphatase regulation, or investigate protein-protein interactions. Its production enables mechanistic insights into pathways governing cell motility, contractility, and apoptosis, highlighting its biomedical relevance.
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