| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPIL6 |
| Uniprot No | Q8IXY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MARPQPCGPP HARCGSPSLP ERPLQVKVVG LFSCPNFQIA KSAAENLKNN HPSKFEDPIL VPLQEFAWHQ YLQEKKRELK NETWEYSSSV ISFVNGQFLG DALDLQKWAH EVWDIVDIKP SALYDALTED FSAKFLRDTK HDFVFLDICI DSSPIGRLIF ELYCDVCPKT CKNFQVLCTG KAGFSQRGIR LHYKNSIFHR IVQNGWIQGG DIVYGKGDNG ESIYGPTFED ENFSVPHNKR GVLGMANKGR HSNGSQFYIT LQATPYLDRK FVAFGQLIEG TEVLKQLELV PTQNERPIHM CRITDSGDPY A |
| 分子量 | 35.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPIL6蛋白的3篇参考文献，涵盖其功能、制备及应用研究：
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1. **文献名称**: *"Peptidyl-Prolyl Isomerase-Like 6 (PPIL6) Promotes Hepatocellular Carcinoma Progression via PI3K/AKT/mTOR Signaling"*
**作者**: Li, Y. et al. (2022)
**摘要**: 本研究通过构建重组人PPIL6蛋白，验证其在肝癌细胞中的促癌作用。实验表明，PPIL6过表达通过激活PI3K/AKT/mTOR通路增强肿瘤增殖和侵袭，重组蛋白的体外功能分析支持其作为潜在治疗靶点。
2. **文献名称**: *"Structural and Functional Characterization of Recombinant Human PPIL6 Reveals Distinct Substrate Specificity"*
**作者**: Martinez, R. & Lee, J. (2020)
**摘要**: 通过大肠杆菌系统表达并纯化重组人PPIL6蛋白，结合X射线晶体学解析其三维结构。功能实验表明，PPIL6对特定含脯氨酸肽段具有异构酶活性，且与Cyclophilin家族其他成员相比具有独特底物偏好。
3. **文献名称**: *"PPIL6 Modulates T Cell Activation via Interaction with CD147: Insights from Recombinant Protein-Based Assays"*
**作者**: Nguyen, T. et al. (2021)
**摘要**: 利用昆虫细胞系统制备重组PPIL6蛋白，研究其与免疫细胞表面蛋白CD147的互作机制。结果显示，PPIL6通过结合CD147调控T细胞活化和炎症因子分泌，提示其在免疫应答中的潜在作用。
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(注：上述文献信息为虚拟示例，若需真实文献，请通过学术数据库检索关键词“recombinant PPIL6”或“PPIL6 protein function”。)
**Background of Recombinant Human PPIL6 Protein**
Recombinant human PPIL6 (Peptidylprolyl Isomerase Like 6) is a synthetically produced protein belonging to the peptidylprolyl isomerase (PPIase) family, which facilitates protein folding by catalyzing the isomerization of proline residues. PPIL6 shares structural homology with cyclophilins but exhibits distinct substrate preferences and functional roles. It is encoded by the *PPIL6* gene, located on human chromosome 6. and is implicated in diverse cellular processes, including transcriptional regulation, spliceosome assembly, and protein trafficking.
Studies suggest PPIL6 interacts with molecular partners in signaling pathways, potentially influencing cell cycle progression, apoptosis, and immune responses. Its involvement in pre-mRNA splicing, via associations with the U5 small nuclear ribonucleoprotein (snRNP) complex, highlights a regulatory role in gene expression. Dysregulation of PPIL6 has been linked to pathological conditions such as cancer, neurodegenerative disorders, and autoimmune diseases, making it a candidate biomarker or therapeutic target.
Recombinant PPIL6 is typically expressed in *E. coli* or mammalian systems, ensuring high purity and bioactivity for experimental use. It serves as a critical tool for elucidating PPIL6’s mechanisms in vitro and in vivo, including structural studies, enzymatic assays, and interaction mapping. Ongoing research aims to clarify its tissue-specific functions and therapeutic potential, bridging gaps in understanding PPIase-related cellular dynamics and disease pathways.
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