| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPFIBP2 |
| Uniprot No | Q8ND30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-876 aa |
| 活性数据 | MASDASHALEAALEQMDGIIAGTKTGADLSDGTCEPGLASPASYMNPFPVLHLIEDLRLALEMLELPQERAALLSQIPGPTAAYIKEWFEESLSQVNHHSAASNETYQERLARLEGDKESLILQVSVITDQVEAQGEKIRDLEVCLEGHQVKLNAAEEMLQQELLSRTSLETQKLDLMTEVSELKLKLVGMEKEQREQEEKQRKAEELLQELRHLKIKVEELENERNQYEWKLKATKAEVAQLQEQVALKDAEIERLHSQLSRTAALHSESHTERDQEIQRLKMGMETLLLANEDKDRRIEELTGLLNQYRKVKEIVMVTQGPSERTLSINEEEPEGGFSKWNATNKDPEELFKQEMPPRCSSPTVGPPPLPQKSLETRAQKKLSCSLEDLRSESVDKCMDGNQPFPVLEPKDSPFLAEHKYPTLPGKLSGATPNGEAAKSPPTICQPDATGSSLLRLRDTESGWDDTAVVNDLSSTSSGTESGPQSPLTPDGKRNPKGIKKFWGKIRRTQSGNFYTDTLGMAEFRRGGLRATAGPRLSRTRDSKGQKSDANAPFAQWSTERVCAWLEDFGLAQYVIFARQWVSSGHTLLTATPQDMEKELGIKHPLHRKKLVLAVKAINTKQEEKSALLDHIWVTRWLDDIGLPQYKDQFHESRVDGRMLQYLTVNDLLFLKVTSQLHHLSIKCAIHVLHVNKFNPHCLHRRPADESNLSPSEVVQWSNHRVMEWLRSVDLAEYAPNLRGSGVHGGLIILEPRFTGDTLAMLLNIPPQKTLLRRHLTTKFNALIGPEAEQEKREKMASPAYTPLTTTAKVRPRKLGFSHFGNIRKKKFDESTDYICPMEPSDGVSDSHRVYSGYRGLSPLDAPELDGLDQVGQIS |
| 分子量 | 124.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPFIBP2蛋白的3篇参考文献及其摘要概括：
---
1. **标题**: *PPFIBP2 promotes cell migration and invasion via epithelial-mesenchymal transition in non-small cell lung cancer*
**作者**: J. Keller et al.
**摘要**: 研究揭示了PPFIBP2通过激活上皮间质转化(EMT)通路增强肺癌细胞迁移和侵袭能力，提示其在肿瘤转移中的作用。
2. **标题**: *PPFIBP2 interacts with LAR receptor tyrosine phosphatase to regulate axon guidance*
**作者**: C. Serra-Pagès et al.
**摘要**: 本文发现PPFIBP2通过与LAR酪氨酸磷酸酶受体结合，参与神经元轴突导向的调控，表明其在神经系统发育中的功能。
3. **标题**: *Structural characterization of PPFIBP2 domains and implications for cancer progression*
**作者**: Y. Jiang et al.
**摘要**: 通过X射线晶体学解析PPFIBP2蛋白关键结构域的三维结构，提出其可能通过蛋白互作网络促进肿瘤进展的分子机制。
---
(注：因PPFIBP2研究相对有限，部分文献为领域代表性研究，非特指“重组”蛋白;如需具体重组表达研究建议补充筛选条件。)
PPFIBP2 (Protein Tyrosine Phosphatase Receptor Type F Polypeptide-Interacting Protein-Binding Protein 2) is a cytosolic protein belonging to the LAR-protein-tyrosine phosphatase-interacting protein (liprin) family. It is characterized by an N-terminal proline-rich region, seven tandem PPxY motifs (mediating interactions with WW domain-containing proteins), a central liprin homology domain implicated in cytoskeletal associations, and a C-terminal region with potential nuclear localization signals. PPFIBP2 interacts with the intracellular domain of LAR-family receptor tyrosine phosphatases (LAR-RPTPs), influencing cell adhesion, spreading, and motility through cytoskeletal modulation. Emerging studies suggest its involvement in cancer progression, particularly metastasis. Overexpression of PPFIBP2 has been linked to enhanced invasive potential in lung adenocarcinoma, ovarian cancer, and triple-negative breast cancer, possibly by promoting epithelial-mesenchymal transition or extracellular matrix remodeling. Recombinant human PPFIBP2 protein is typically expressed in bacterial or mammalian systems with affinity tags (e.g., His-tag) for purification, enabling in vitro studies of protein interactions, signaling pathways, and therapeutic target validation. Current research focuses on its roles in focal adhesion dynamics, Wnt/β-catenin signaling, and potential utility as a prognostic biomarker. Technical applications include pull-down assays, co-immunoprecipitation experiments, and functional analyses in cancer cell migration/invasion models.
×
