| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPFIA4 |
| Uniprot No | O75335 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-701 aa |
| 活性数据 | MGSAADVRFS LGTTTHAPPG VHRRYSALRE ESAKDWETSP LPGMLAPAAG PAFDSDPEIS DVDEDEPGGL VGSADVVSPS GHSDAQTLAM MLQEQLDAIN EEIRMIQEEK ESTELRAEEI ETRVTSGSME ALNLKQLRKR GSIPTSLTAL SLASASPPLS GRSTPKLTSR SAAQDLDRMG VMTLPSDLRK HRRKLLSPVS REENREDKAT IKCETSPPSS PRTLRLEKLG HPALSQEEGK SALEDQGSNP SSSNSSQDSL HKGAKRKGIK SSIGRLFGKK EKGRLIQLSR DGATGHVLLT DSEFSMQEPM VPAKLGTQAE KDRRLKKKHQ LLEDARRKGM PFAQWDGPTV VSWLELWVGM PAWYVAACRA NVKSGAIMSA LSDTEIQREI GISNALHRLK LRLAIQEMVS LTSPSAPPTS RTSSGNVWVT HEEMETLETS TKTDSEEGSW AQTLAYGDMN HEWIGNEWLP SLGLPQYRSY FMECLVDARM LDHLTKKDLR VHLKMVDSFH RTSLQYGIMC LKRLNYDRKE LEKRREESQH EIKDVLVWTN DQVVHWVQSI GLRDYAGNLH ESGVHGALLA LDENFDHNTL ALILQIPTQN TQARQVMERE FNNLLALGTD RKLDDGDDKV FRRAPSWRKR FRPREHHGRG GMLSASAETL PAGFRVSTLG TLQPPPAPPK KIMPEAHSHY LYGHMLSAFR D |
| 分子量 | 134.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPFIA4蛋白的3条示例性参考文献摘要(内容为示例性质，非真实文献)：
1. **文献名称**：*PPFIA4 regulates focal adhesion formation and cancer cell migration via integrin signaling*
**作者**：Smith et al.
**摘要**：研究揭示了重组人PPFIA4蛋白通过与整合素β1亚基结合调控黏着斑形成，促进肿瘤细胞迁移的分子机制。
2. **文献名称**：*Structural basis of PPFIA4 tyrosine phosphorylation in synaptic scaffolding*
**作者**：Lee & Zhang
**摘要**：利用重组PPFIA4蛋白进行X射线晶体学分析，揭示了其酪氨酸磷酸化位点对结合LAR-RPTP等突触支架蛋白的关键作用。
3. **文献名称**：*PPFIA4 overexpression enhances chemoresistance in colorectal cancer by modulating ERK pathway*
**作者**：Wang et al.
**摘要**：通过重组PPFIA4蛋白实验发现，其在结直肠癌中过表达可激活ERK信号通路，导致奥沙利铂耐药性增强。
(注：以上文献为模拟示例，实际文献需通过PubMed或学术数据库检索。)
**Recombinant human PPFIA4 protein** is a genetically engineered form of the PPFIA4 (PTPRF Interacting Protein Alpha 4) protein, a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. PPFIA4 plays critical roles in cell adhesion, cytoskeletal organization, and intracellular signaling by interacting with membrane-associated proteins like LAR-PTPRF. Structurally, it contains coiled-coil domains and a C-terminal PDZ-binding motif, facilitating protein-protein interactions.
Functionally, PPFIA4 is implicated in regulating synaptic development, cell migration, and receptor clustering. Dysregulation of PPFIA4 has been linked to cancer progression, particularly in promoting tumor invasion, metastasis, and therapy resistance. Studies suggest its overexpression in certain cancers enhances signaling pathways like MAPK/ERK, contributing to aggressive phenotypes.
Recombinant PPFIA4 is produced using expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and bioactivity. It serves as a tool for studying PPFIA4's molecular mechanisms, identifying binding partners, and exploring its role in diseases. Researchers also utilize it to develop targeted therapies or diagnostic biomarkers for cancers with PPFIA4 dysregulation. Its study offers insights into cell signaling networks and potential clinical applications.
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