| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPEF2 |
| Uniprot No | O14830 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-753 aa |
| 活性数据 | MGSGTSTQHH FAFQNAERAF KAAALIQRWY RRYVARLEMR RRCTWSIFQS IEYAGQQDQV KLHDFFSYLM DHFIPSSHND RDFLTRIFTE DRFAQDSEMK KCSDYESIEV PDSYTGPRLS FPLLPDHATA LVEAFRLKQQ LHARYVLNLL YETKKHLVQL PNINRVSTCY SEEITVCGDL HGQLDDLIFI FYKNGLPSPE RSYVFNGDFV DRGKDSVEIL MILFAFMLVY PKEFHLNRGN HEDHMVNLRY GFTKEVMNKY KVHGKEILRT LQDVFCWLPL ATLIDEKVLI LHGGVSDITD LELLDKIERS KIVSTMRCKT RQKSEKQMEE KRRANQKSSA QGPIPWFLPE SRSLPSSPLR LGSYKAQKTS RSSSIPCSGS LDGRELSRQV RSSVELELER CRQQAGLLVT GEKEEPSRSA SEADSEAGEL RKPTQEEWRQ VVDILWSDPM AQEGCKANTI RGGGCYFGPD VTQQLLQKYN MQFLIRSHEC KPEGYEFCHN RKVLTIFSAS NYYEVGSNRG AYVKLGPALT PHIVQYQANK VTHTLTMRQR ISRVEESALR ALREKLFAHS SDLLSEFKKH DADKVGLITL SDWAAAVESV LHLGLPWRML RPQLVNSSAD NMLEYKSWLK NLAKEQLSRE NIQSSLLETL YRNRSNLETI FRIIDSDHSG FISLDEFRQT WKLFSSHMNI DITDDCICDL ARSIDFNKDG HIDINEFLEA FRLVEKSCPE GDASECPQAT NAKDSGCSSP GAH |
| 分子量 | 86.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPEF2蛋白的3篇参考文献的简要信息：
1. **文献名称**：*Characterization of PPEF2. a protein phosphatase with roles in neuronal differentiation*
**作者**：Smith J, et al.
**摘要**：本研究首次表达了重组人PPEF2蛋白，发现其在HEK293细胞中通过哺乳动物表达系统可溶性表达，并具有钙依赖的磷酸酶活性。实验表明PPEF2可能通过调节MAPK信号通路参与神经细胞分化。
2. **文献名称**：*Structural insights into the catalytic mechanism of human PPEF2*
**作者**：Li X, Wang Y.
**摘要**：通过X射线晶体学解析了重组人PPEF2蛋白的催化结构域，揭示了其与钙离子结合的关键EF-hand结构域，并对底物特异性进行了分析，提出了PPEF2在视网膜光信号转导中的潜在作用。
3. **文献名称**：*PPEF2 knockout and overexpression models reveal its role in cell cycle regulation*
**作者**：Garcia-R et al.
**摘要**：构建了重组PPEF2过表达和敲除的Hela细胞模型，发现PPEF2通过去磷酸化CDK1调控G2/M期转换，暗示其在癌症细胞增殖中的调控功能。蛋白纯化采用His标签亲和层析，活性通过体外磷酸酶实验验证。
注：上述文献信息为示例性质，实际研究中请通过学术数据库(如PubMed)以“PPEF2”或“Protein Phosphatase EF-Hand 2”为关键词检索最新文献。
**Recombinant Human PPEF2 Protein: Background**
Recombinant human PPEF2 (Protein Phosphatase EF-Hand Domain Containing 2) is a calcium-regulated serine/threonine phosphatase belonging to the PPEF subfamily within the PPM phosphatase family. The PPEF2 gene, located on chromosome 4q21.22. encodes a protein containing conserved EF-hand motifs that bind calcium ions, enabling its regulation by calcium signaling pathways. PPEF2 is primarily expressed in sensory tissues, including the retina and testis, suggesting roles in specialized cellular processes.
Functionally, PPEF2 is implicated in dephosphorylating key substrates involved in signal transduction, though its specific targets remain under investigation. Studies highlight its potential involvement in visual signal transduction, neuronal development, and circadian rhythm modulation. Unlike its paralog PPEF1. which is retina-specific, PPEF2 shows broader tissue distribution but shares structural similarities, including catalytic domains critical for phosphatase activity.
Recombinant PPEF2 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for biochemical and structural studies. Its recombinant form allows researchers to explore enzymatic kinetics, calcium dependency, and interactions with regulatory proteins. Dysregulation of PPEF2 has been loosely associated with neurodegenerative diseases and cancer, though mechanistic insights are limited. Current research focuses on elucidating its physiological roles and therapeutic potential, leveraging recombinant tools to decode its contribution to calcium-mediated cellular pathways.
This protein remains a subject of interest for understanding phosphatase diversity and calcium-dependent signaling in health and disease.
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