| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPARBP |
| Uniprot No | Q15648 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 878-1031 aa |
| 活性数据 | FGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPF |
| 分子量 | 32.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PPARBP(MED1/TRAP220)蛋白的3条参考文献示例(内容为模拟概括，建议通过学术数据库核对原文)：
1. **文献名称**: *"MED1/TRAP220 in Adipogenesis and PPARγ Transcriptional Activation"*
**作者**: Yuan CX, et al.
**摘要**: 研究揭示了MED1(即PPARBP)作为中介体复合物核心组分，通过与PPARγ直接结合调控脂肪生成。实验表明，重组MED1蛋白在体外增强PPARγ依赖的转录活性，敲低MED1导致脂肪分化受阻。
2. **文献名称**: *"Structural Basis for PPARγ Activation by MED1 in Lipid Metabolism"*
**作者**: Kang HS, et al.
**摘要**: 通过X射线晶体学解析了重组人MED1蛋白的PPARγ结合域结构，阐明其特异性相互作用机制。结果揭示了MED1与PPARγ/LXR异源二聚体的结合模式，为代谢疾病药物靶点提供依据。
3. **文献名称**: *"Essential Role of MED1 in Mouse Embryonic Development and PPAR Signaling"*
**作者**: Kadowaki T, et al.
**摘要**: 利用基因敲除模型发现，MED1缺失导致胚胎致死，并破坏PPARα、PPARγ等受体介导的基因表达。重组MED1蛋白的功能拯救实验证实其在脂代谢和能量平衡中的核心调控作用。
**注意**：以上信息为简化示例，具体文献需通过PubMed/Google Scholar以“MED1”、“TRAP220”或“PPARBP”为关键词检索确认。
Peroxisome proliferator-activated receptor-binding protein (PPARBP), also known as mediator complex subunit 1 (MED1), is a critical transcriptional coactivator that facilitates communication between gene-specific regulatory proteins and the RNA polymerase II machinery. It serves as a key component of the mediator complex, a multi-protein assembly essential for regulating RNA polymerase II-dependent transcription. PPARBP directly interacts with nuclear receptors, including peroxisome proliferator-activated receptors (PPARs), thyroid hormone receptors, and estrogen receptors, modulating their target gene expression.
Originally identified for its role in PPAR signaling, PPARBP is involved in diverse physiological processes, including lipid metabolism, glucose homeostasis, cell differentiation, and energy balance. Studies link its dysfunction to metabolic disorders, cancer, and inflammatory diseases. For example, reduced PPARBP expression correlates with insulin resistance, while its overexpression may influence tumor progression by altering oncogenic pathways.
Recombinant PPARBP protein, produced via bacterial or mammalian expression systems, enables detailed functional and structural studies. It is widely used to investigate protein-protein interactions, nuclear receptor activation mechanisms, and transcriptional regulation. The recombinant form also holds therapeutic potential for developing targeted treatments for metabolic syndromes or cancers linked to PPAR signaling aberrations. Its high purity and controlled production enhance reproducibility in biochemical assays and drug discovery platforms.
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