| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POU6F1 |
| Uniprot No | Q14863 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-301 aa |
| 活性数据 | MPGISSQILT NAQGQVIGTL PWVVNSASVA APAPAQSLQV QAVTPQLLLN AQGQVIATLA SSPLPPPVAV RKPSTPESPA KSEVQPIQPT PTVPQPAVVI ASPAPAAKPS ASAPIPITCS ETPTVSQLVS KPHTPSLDED GINLEEIREF AKNFKIRRLS LGLTQTQVGQ ALTATEGPAY SQSAICRFEK LDITPKSAQK LKPVLEKWLN EAELRNQEGQ QNLMEFVGGE PSKKRKRRTS FTPQAIEALN AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQI P |
| 分子量 | 63.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人POU6F1蛋白研究的参考文献概括(注：以下内容为假设性举例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Expression and Functional Analysis of Recombinant Human POU6F1 in Neuronal Differentiation"
**作者**: Smith A. et al. (2018)
**摘要**: 本研究通过大肠杆菌系统成功表达了重组人POU6F1蛋白，证实其通过调控靶基因(如NeuroD1)促进神经元分化，揭示了其在神经发育中的关键作用。
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2. **文献名称**: "Structural Insights into the DNA-Binding Domain of POU6F1 via X-ray Crystallography"
**作者**: Lee H. et al. (2020)
**摘要**: 利用重组POU6F1蛋白的DNA结合结构域进行X射线晶体学分析，解析了其与特定DNA序列(如ATGCAAAT)的相互作用机制，为理解其转录调控功能提供结构基础。
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3. **文献名称**: "POU6F1 Acts as a Tumor Suppressor in Breast Cancer by Modulating Epithelial-Mesenchymal Transition"
**作者**: Zhang Y. et al. (2021)
**摘要**: 通过体外实验验证重组POU6F1蛋白过表达可抑制乳腺癌细胞增殖和转移，机制涉及抑制Wnt/β-catenin信号通路及调控EMT相关基因(如E-cadherin)。
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**备注**：上述内容为示例，建议通过PubMed或Google Scholar以关键词“POU6F1 recombinant protein”或“POU6F1 function”检索真实文献。
**Background of Recombinant Human POU6F1 Protein**
The POU6F1 protein, encoded by the *POU6F1* gene, belongs to the POU transcription factor family, characterized by a conserved DNA-binding domain (POU domain) comprising a POU-specific region and a homeodomain. This dual-domain structure enables sequence-specific DNA binding and regulation of target gene expression. POU6F1 plays critical roles in development and cellular differentiation, particularly in neural, ocular, and renal tissues. Studies highlight its involvement in neurodevelopment, eye morphogenesis, and kidney tubule formation. Dysregulation of POU6F1 has been linked to developmental disorders, optic nerve hypoplasia, and certain cancers, suggesting its tumor-suppressive or oncogenic roles depending on context.
Recombinant human POU6F1 protein is produced via *in vitro* expression systems (e.g., bacterial, mammalian, or insect cells), ensuring high purity and activity for functional studies. Researchers utilize it to investigate DNA-protein interactions, signaling pathways (e.g., Wnt/β-catenin), and regulatory networks in disease models. Its applications extend to drug discovery, biomarker development, and mechanistic studies of POU6F1-associated pathologies. Despite progress, the full spectrum of its biological functions and clinical relevance remains under exploration, necessitating further research to unravel its therapeutic potential.
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