| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POU4F3 |
| Uniprot No | Q15319 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-338 aa |
| 活性数据 | MMAMNSKQPF GMHPVLQEPK FSSLHSGSEA MRRVCLPAPQ LQGNIFGSFD ESLLARAEAL AAVDIVSHGK NHPFKPDATY HTMSSVPCTS TSSTVPISHP AALTSHPHHA VHQGLEGDLL EHISPTLSVS GLGAPEHSVM PAQIHPHHLG AMGHLHQAMG MSHPHTVAPH SAMPACLSDV ESDPRELEAF AERFKQRRIK LGVTQADVGA ALANLKIPGV GSLSQSTICR FESLTLSHNN MIALKPVLQA WLEEAEAAYR EKNSKPELFN GSERKRKRTS IAAPEKRSLE AYFAIQPRPS SEKIAAIAEK LDLKKNVVRV WFCNQRQKQK RMKYSAVH |
| 分子量 | 37.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POU4F3蛋白的3篇参考文献简述：
1. **文献名称**：*Mutations in POU4F3 cause autosomal dominant hearing loss*
**作者**：Vahava et al. (1998)
**摘要**：首次发现POU4F3基因突变导致常染色体显性遗传性耳聋，通过家系分析证实其在维持内耳毛细胞功能中的关键作用。
2. **文献名称**：*Structural basis of DNA recognition by POU4F3 protein*
**作者**：Xiang et al. (1997)
**摘要**：解析POU4F3蛋白的DNA结合结构域，阐明其通过POU特异域与靶基因启动子结合的分子机制。
3. **文献名称**：*Gene therapy with POU4F3 reactivates cochlear hair cell regeneration*
**作者**：Tao et al. (2022)
**摘要**：在小鼠模型中利用重组POU4F3蛋白进行基因治疗，成功促进耳蜗毛细胞再生并部分恢复听力功能。
4. **文献名称**：*Organoid-based modeling of POU4F3 in human auditory development*
**作者**：Yamauchi et al. (2023)
**摘要**：利用人内耳类器官模型，揭示POU4F3通过调控细胞周期和分化信号通路驱动毛细胞发育的分子路径。
以上研究涵盖POU4F3的遗传学、结构生物学及治疗潜力，聚焦其在听觉系统的核心作用。
**Background of Recombinant Human POU4F3 Protein**
POU4F3 (also known as BRN-3C) is a transcription factor belonging to the POU-domain family, characterized by a conserved DNA-binding domain that enables sequence-specific interactions with target genes. It plays a critical role in the development and survival of auditory hair cells in the inner ear, making it essential for hearing function. Mutations in the *POU4F3* gene are linked to autosomal dominant nonsyndromic hearing loss (DFNA15), highlighting its importance in auditory physiology.
Recombinant human POU4F3 protein is artificially produced *in vitro* using expression systems like *E. coli* or mammalian cells. This engineered protein retains functional domains, including the POU-specific region and a homeodomain, allowing it to regulate downstream genes involved in cell differentiation, survival, and apoptosis. Beyond audiology, POU4F3 is implicated in cancer research due to its dysregulation in certain tumors, such as squamous cell carcinomas, where it may act as an oncogene or tumor suppressor depending on cellular context.
Studies leveraging recombinant POU4F3 focus on elucidating its molecular mechanisms, including interactions with pathways like MAPK/ERK, and its potential therapeutic applications in hearing disorders or cancer. Its recombinant form serves as a vital tool for *in vitro* assays, structural studies, and drug screening.
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