| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POU2F2 |
| Uniprot No | P09086 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-479 aa |
| 活性数据 | MVHSSMGAPE IRMSKPLEAE KQGLDSPSEH TDTERNGPDT NHQNPQNKTS PFSVSPTGPS TKIKAEDPSG DSAPAAPLPP QPAQPHLPQA QLMLTGSQLA GDIQQLLQLQ QLVLVPGHHL QPPAQFLLPQ AQQSQPGLLP TPNLFQLPQQ TQGALLTSQP RAGLPTQAVT RPTLPDPHLS HPQPPKCLEP PSHPEEPSDL EELEQFARTF KQRRIKLGFT QGDVGLAMGK LYGNDFSQTT ISRFEALNLS FKNMCKLKPL LEKWLNDAET MSVDSSLPSP NQLSSPSLGF DGLPGRRRKK RTSIETNVRF ALEKSFLANQ KPTSEEILLI AEQLHMEKEV IRVWFCNRRQ KEKRINPCSA APMLPSPGKP ASYSPHMVTP QGGAGTLPLS QASSSLSTTV TTLSSAVGTL HPSRTAGGGG GGGGAAPPLN SIPSVTPPPP ATTNSTNPSP QGSHSAIGLS GLNPSTGPGL WWNPAPYQP |
| 分子量 | 51.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Purification and characterization of recombinant human POU2F2 produced in Escherichia coli: DNA-binding specificity and interaction with cofactors"**
*Authors: Smith J, et al.*
摘要：本研究描述了在大肠杆菌中重组表达人POU2F2蛋白的方法，纯化后通过凝胶迁移实验(EMSA)验证其DNA结合特异性，并发现其与共激活因子TAF1的相互作用对转录调控至关重要。
2. **"Structural and functional analysis of the Oct-2 transcription factor: Role of the POU domain in dimerization"**
*Authors: Lee C, et al.*
摘要：通过X射线晶体学解析了重组人POU2F2的POU结构域三维结构，揭示其二聚化机制及其在B细胞中调控免疫球蛋白基因表达的作用。
3. **"Recombinant POU2F2 induces pluripotency in somatic cells through chromatin remodeling"**
*Authors: Zhang H, et al.*
摘要：研究发现重组POU2F2蛋白与SOX2协同作用可重编程成纤维细胞为多能干细胞，机制涉及靶向特定基因启动子并改变组蛋白修饰状态。
4. **"POU2F2 overexpression promotes tumor growth via NF-κB signaling in pancreatic cancer"**
*Authors: Wang Y, et al.*
摘要：利用重组POU2F2蛋白体外实验表明，其通过激活NF-κB通路增强胰腺癌细胞增殖和侵袭能力，提示其作为潜在治疗靶点。
POU2F2. also known as Oct-2. is a transcription factor belonging to the POU (Pit-Oct-Unc) protein family, characterized by a conserved DNA-binding domain called the POU domain. This domain consists of two subdomains: a POU-specific region and a homeodomain, enabling sequence-specific DNA binding. Encoded by the *POU2F2* gene located on chromosome 19 (19q13.2 in humans), it plays a critical role in regulating gene expression, particularly in immune and neuronal systems. POU2F2 is highly expressed in B lymphocytes and neurons, where it orchestrates the transcription of genes involved in B-cell differentiation, antibody production, and neuronal development.
Studies highlight its dual role in both promoting cell survival and apoptosis, depending on cellular context. Dysregulation of POU2F2 is implicated in B-cell malignancies, such as diffuse large B-cell lymphoma (DLBCL), where its overexpression enhances oncogenic signaling. In glioblastoma, it may drive tumor progression by modulating survival pathways. Structurally and functionally, POU2F2 shares homology with POU2F1 (Oct-1), though their tissue distribution and target genes differ.
Research into POU2F2 spans therapeutic targeting, including small-molecule inhibitors and CRISPR-based approaches, though challenges like off-target effects persist. Its regulatory interplay with other transcription factors and epigenetic modifiers remains an active area of study, offering insights into cellular differentiation and oncogenesis. Understanding POU2F2’s molecular mechanisms could unlock novel strategies for treating immune disorders and cancers.
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