| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POTEA |
| Uniprot No | Q6S8J7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-498 aa |
| 活性数据 | MVAEVSPKLA ASPMKKPFGF RGKMGKWCCC CFPCCRGSGK NNMGAWRDHD DSAFTEPRYH VRREDLGKLH RAAWWGEVPR ADLIVMLRGP GINKRDKKKR TALHLACANG NSEVVSLLLD RQCQLHVFDS KKRTALIKAV QCQEDECALM LLQHGTDPNL PDMYGNTALH YAVYNEDKLM AKTLLLYGAD IESKNKGGLT PLLLAVHGQK QRMVKFLIKK KANLNALDRF GRTALILAVR CGSASIVSLL LQQNIDVFSQ DVFGQTAEDY AVSSHHSIIC QLLSDYKENQ MPNNSSGNSN PEQDLKLTSE EEPQRLKGSE NSQHEKVTQE PDINKDCDRE VEEEMQKHGS NNVGLSENLT DGAAAGNGDG GLVPQRKSRK HENQQFPNTE IEEYHRPEKK SNEKNKVKSQ IHSVDNLDDI TWPSEIASED YDLLFSNYET FTLLIEQLKM DFNDSASLSK IQDAVISEEH LLELKNSHYE QLTVEVEQME NMVHVLQK |
| 分子量 | 56.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POTEA蛋白的模拟参考文献示例(请注意，POTEA相关研究较少，以下信息为学术示例格式，具体文献需通过数据库验证)：
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1. **文献名称**: "Expression and Functional Analysis of Recombinant Human POTEA in Cancer Cell Lines"
**作者**: Chen L, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人POTEA蛋白，并发现其在多种癌细胞系中调控细胞凋亡通路，可能通过影响Bcl-2家族蛋白活性发挥作用。
2. **文献名称**: "Structural Characterization of POTEA Protein Using Cryo-EM"
**作者**: Gupta R, et al.
**摘要**: 首次通过冷冻电镜解析了POTEA蛋白的三维结构，揭示了其潜在的跨膜结构域和磷酸化位点，为研究其在信号转导中的作用提供结构基础。
3. **文献名称**: "POTEA as a Novel Biomarker in Autoimmune Diseases: Recombinant Protein-Based Assay Development"
**作者**: Kim S, Park JH.
**摘要**: 基于重组POTEA蛋白开发了ELISA检测方法，发现其在类风湿性关节炎患者血清中显著高表达，提示其作为疾病生物标志物的潜力。
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**建议**：实际研究中请通过PubMed、Web of Science等数据库，以“recombinant POTEA”或“POTEA protein function”为关键词检索最新文献，部分研究可能聚焦于POTE基因家族其他成员(如POTEB/POTEC)。
Recombinant human POTEA protein is a genetically engineered form of the POTEA (Partner of TEA domain-containing transcription factor) protein, which plays a role in transcriptional regulation and cellular signaling pathways. The POTEA gene encodes a protein that interacts with TEA domain (TEAD) transcription factors, key mediators of the Hippo signaling pathway. This pathway is critical for regulating organ size, cell proliferation, apoptosis, and stem cell self-renewal. Dysregulation of Hippo signaling is implicated in cancers and developmental disorders.
The native POTEA protein is believed to modulate TEAD activity by influencing its interaction with co-activators like YAP/TAZ, thereby affecting gene expression profiles. However, the precise molecular mechanisms and physiological functions of POTEA remain under investigation. Recombinant POTEA is typically produced in heterologous systems (e.g., Escherichia coli or mammalian cell cultures) using DNA recombination techniques. This allows large-scale production of purified protein for functional studies, structural analysis, and drug discovery efforts. Researchers employ it to explore its binding partners, post-translational modifications, and regulatory roles in Hippo pathway dynamics. Its recombinant form is particularly valuable for standardized in vitro assays, antibody development, and screening therapeutic compounds targeting Hippo-associated diseases. Current challenges include elucidating its tissue-specific functions and clarifying conflicting reports about its pro- or anti-tumorigenic effects in different cancer contexts.
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