| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POP5 |
| Uniprot No | Q969H6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-163 aa |
| 活性数据 | MVRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNAYTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRRQLLILLQNCTDEGEREAIQKSVTRSCLLEEEEESGEEAAEAME |
| 分子量 | 45.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POP5蛋白的3-4篇文献示例(部分信息基于公开研究背景整理)：
1. **文献名称**：*Structural Insights into the Role of POP5 in Human Ribonuclease P*
**作者**：Karasawa, S. et al.
**摘要**：研究了重组人POP5蛋白在核糖核酸酶P(RNase P)复合体中的结构与功能，通过X射线晶体学揭示了POP5与其他亚基(如RPP14)的相互作用机制，阐明了其在RNA加工中的催化辅助作用。
2. **文献名称**：*Recombinant Expression and Functional Characterization of Human POP5 in tRNA Processing*
**作者**：Lopez, M.D. & Li, Y.
**摘要**：报道了人POP5蛋白在大肠杆菌中的重组表达与纯化方法，并通过体外酶活实验证明POP5对RNase P复合体的稳定性及pre-tRNA底物切割能力的关键影响。
3. **文献名称**：*POP5 as a Novel Therapeutic Target: Biophysical and Biochemical Analysis*
**作者**：Gopalan, V. et al.
**摘要**：分析了重组POP5蛋白的热稳定性和构象变化，探讨其与疾病(如癌症)中RNA代谢异常的关联，提出POP5可能成为药物开发的潜在靶点。
4. **文献名称**：*Evolutionary Conservation of POP5 in Eukaryotic RNase P Complexes*
**作者**：Esakova, O. & Krasilnikov, A.S.
**摘要**：通过对比人类、酵母等物种的重组POP5蛋白序列和功能，揭示了其在真核生物RNase P复合体进化中的高度保守性及其对RNA识别的重要性。
注：以上文献信息为示例，实际引用需以具体论文为准。建议通过PubMed或Web of Science以“human POP5 protein”、“RNase P subunit”等关键词检索最新研究。
Recombinant human POP5 protein, a key component of the ribonuclease MRP (RNase MRP) complex, plays an essential role in ribosomal RNA processing and cell cycle regulation. The endogenous POP5 protein is encoded by the POP5 gene located on human chromosome 12q24.31 and functions as a non-catalytic subunit within the evolutionarily conserved RNase MRP complex. This ribonucleoprotein complex shares structural similarities with RNase P but has distinct biological functions, primarily cleaving precursor rRNA in the nucleolus to facilitate 5.8S rRNA maturation and regulating cyclin B2 mRNA degradation during mitosis.
The recombinant version is typically produced using heterologous expression systems like *E. coli* or mammalian cell cultures, enabling controlled production for research applications. Structural studies reveal POP5 contains characteristic protein domains that facilitate RNA binding and complex stabilization. Its recombinant form has become instrumental in studying ribosome biogenesis defects linked to human genetic disorders such as cartilage-hair hypoplasia, where RNase MRP mutations are implicated. Researchers utilize recombinant POP5 to investigate RNA-protein interactions, enzyme kinetics, and pathological mechanisms through *in vitro* reconstitution experiments. The protein's biochemical properties also make it valuable for developing molecular tools in nucleic acid research. Current investigations focus on its potential roles beyond rRNA processing, including emerging connections to cellular stress responses and cancer biology. Production typically includes affinity tags (e.g., His-tag) for purification, ensuring high purity (>95%) for structural and functional analyses.
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