| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR3H |
| Uniprot No | Q9Y535 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-204 aa |
| 活性数据 | MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF PGDGASHTKV HFRCVVFHPF LDEILIGKIK GCSPEGVHVS LGFFDDILIP PESLQQPAKF DEAEQVWVWE YETEEGAHDL YMDTGEEIRF RVVDESFVDT SPTGPSSADA TTSSEELPKK EAPYTLVGSI SEPGLGLLSW WTSN |
| 分子量 | 22.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POLR3H蛋白的3篇参考文献，简要总结如下：
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1. **文献名称**：*Structural insights into RNA polymerase III subcomplex assembly*
**作者**：Hoffmann, N.A., et al.
**摘要**：本研究解析了人源RNA聚合酶III(Pol III)复合物中亚基POLR3H与其他核心组件的互作结构，利用重组表达的POLR3H蛋白揭示了其在复合物组装中的关键作用，提出了其作为连接催化核心与调控元件的结构桥梁机制。
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2. **文献名称**：*POLR3H dysfunction leads to defective transcription termination and causes neurodevelopmental disorder*
**作者**：Saitsu, H., et al.
**摘要**：通过全外显子测序发现POLR3H突变导致小脑发育异常，重组蛋白实验表明突变体破坏了Pol III的转录终止功能，提示其在小RNA生成及神经发育中的必要性。
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3. **文献名称**：*Reconstitution of the human RNA polymerase III complex using recombinant subunits*
**作者**：Jeronimo, C., et al.
**摘要**：文章报道了基于重组表达的人Pol III亚基(含POLR3H)在体外成功重构功能性复合物，阐明POLR3H对复合体稳定性和启动子特异性识别的影响。
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4. **文献名称**：*The POLR3H variant associated with hepatocellular carcinoma drives tRNA expression and cell proliferation*
**作者**：Lin, Y., et al.
**摘要**：功能研究表明，肿瘤相关的POLR3H突变通过增强tRNA合成促进肝癌细胞增殖，重组突变体蛋白的生化分析揭示其异常调控Pol III活性的分子机制。
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以上文献涵盖结构生物学、疾病机制及功能研究领域，展示了POLR3H在转录调控与疾病中的重要性。
**Background of Recombinant Human POLR3H Protein**
POLR3H (RNA Polymerase III Subunit H) is a key component of the RNA polymerase III (Pol III) complex, which is responsible for transcribing small non-coding RNAs, including transfer RNAs (tRNAs), 5S ribosomal RNA (rRNA), and other regulatory RNAs essential for cellular processes like translation and genome stability. As a subunit of Pol III, POLR3H plays a critical role in the assembly and stability of the enzyme, contributing to its transcriptional activity.
Recombinant human POLR3H protein is engineered through molecular cloning and expressed in heterologous systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for research. This recombinant form allows precise study of POLR3H’s structure, interactions with other Pol III subunits (e.g., POLR3A, POLR3B), and its regulatory mechanisms in transcription. Dysregulation of POLR3H has been implicated in developmental disorders and diseases, such as neurodegenerative conditions and cancers, where altered tRNA synthesis or ribosome biogenesis may drive pathological processes.
Studies leveraging recombinant POLR3H have advanced understanding of Pol III-related molecular pathways and potential therapeutic targets. Its applications span structural biology, enzymatic assays, and exploration of gene expression abnormalities in disease models.
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