| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR3E |
| Uniprot No | Q9NVU0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-708 aa |
| 活性数据 | MANEEDDPVV QEIDVYLAKS LAEKLYLFQY PVRPASMTYD DIPHLSAKIK PKQQKVELEM AIDTLNPNYC RSKGEQIALN VDGACADETS TYSSKLMDKQ TFCSSQTTSN TSRYAAALYR QGELHLTPLH GILQLRPSFS YLDKADAKHR EREAANEAGD SSQDEAEDDV KQITVRFSRP ESEQARQRRV QSYEFLQKKH AEEPWVHLHY YGLRDSRSEH ERQYLLCPGS SGVENTELVK SPSEYLMMLM PPSQEEEKDK PVAPSNVLSM AQLRTLPLAD QIKILMKNVK VMPFANLMSL LGPSIDSVAV LRGIQKVAML VQGNWVVKSD ILYPKDSSSP HSGVPAEVLC RGRDFVMWKF TQSRWVVRKE VATVTKLCAE DVKDFLEHMA VVRINKGWEF ILPYDGEFIK KHPDVVQRQH MLWTGIQAKL EKVYNLVKET MPKKPDAQSG PAGLVCGDQR IQVAKTKAQQ NHALLERELQ RRKEQLRVPA VPPGVRIKEE PVSEEGEEDE EQEAEEEPMD TSPSGLHSKL ANGLPLGRAA GTDSFNGHPP QGCASTPVAR ELKAFVEATF QRQFVLTLSE LKRLFNLHLA SLPPGHTLFS GISDRMLQDT VLAAGCKQIL VPFPPQTAAS PDEQKVFALW ESGDMSDQHR QVLLEIFSKN YRVRRNMIQS RLTQECGEDL SKQEVDKVLK DCCVSYGGMW YLKGTVQS |
| 分子量 | 79.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人POLR3E蛋白相关的3篇参考文献及其摘要要点：
1. **文献名称**: *Structural insights into human RNA polymerase III*
**作者**: Girbig, M. et al. (2021)
**摘要**: 通过冷冻电镜解析人RNA聚合酶III(POLR3)全酶结构，揭示了其亚基组成(包括POLR3E)在tRNA转录中的作用，重组表达的POLR3E被用于复合体组装实验。
2. **文献名称**: *Mutations in POLR3E impair mitochondrial RNA splicing and cause developmental disorders*
**作者**: Saoura, M. et al. (2020)
**摘要**: 研究报道POLR3E基因突变与神经发育异常相关，通过重组表达突变体蛋白，发现其导致RNA聚合酶III功能缺陷和线粒体RNA剪接异常。
3. **文献名称**: *Recombinant expression and functional characterization of human RNA polymerase III subunits*
**作者**: Wu, L. et al. (2018)
**摘要**: 描述了重组表达人POLR3E及其相邻亚基的方法，发现POLR3E在稳定POLR3复合体及招募转录因子中的关键作用。
POLR3E, also known as RPC5. is a subunit of human RNA polymerase III (Pol III), a multi-protein complex responsible for transcribing small non-coding RNAs, including transfer RNAs (tRNAs), 5S ribosomal RNA, and other regulatory RNAs. Encoded by the POLR3E gene located on chromosome 16q12.2. this protein plays a critical role in Pol III assembly and transcriptional specificity. Structurally, POLR3E contains a tetratricopeptide repeat (TPR) domain, which facilitates protein-protein interactions within the Pol III holoenzyme.
As a recombinant protein, POLR3E is artificially expressed in vitro (e.g., in E. coli or mammalian systems) for functional studies. Researchers utilize recombinant POLR3E to dissect Pol III’s mechanistic roles in fundamental processes like cell growth, differentiation, and stress responses. Dysregulation of POLR3E has been implicated in diseases, including neurodevelopmental disorders and cancers, due to altered tRNA synthesis affecting protein translation. Recent studies also explore its potential as a biomarker or therapeutic target. Recombinant forms enable structural analysis (e.g., cryo-EM) and interaction mapping with transcription factors (e.g., MAF1), advancing our understanding of Pol III regulation in health and disease.
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