| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR3D |
| Uniprot No | P05423 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-398 aa |
| 活性数据 | SEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP NIISRKIKEE PKEEVTVKKE KRERDRDRQR EGHGRGRGRP EVIQSHSIFE QGPAEMMKKK GNWDKTVDVS DMGPSHIINI KKEKRETDEE TKQILRMLEK DDFLDDPGLR NDTRNMPVQL PLAHSGWLFK EENDEPDVKP WLAGPKEEDM EVDIPAVKVK EEPRDEEEEA KMKAPPKAAR KTPGLPKDVS VAELLRELSL TKEEELLFLQ LPDTLPGQPP TQDIKPIKTE VQGEDGQVVL IKQEKDREAK LAENACTLAD LTEGQVGKLL IRKSGRVQLL LGKVTLDVTM GTACSFLQEL VSVGLGDSRT GEMTVLGHVK HKLVCSPDFE SLLDHKHR |
| 分子量 | 44.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POLR3D蛋白的3篇参考文献(示例为虚拟合成内容，仅供参考)：
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1. **文献名称**：Structural insights into human RNA polymerase III subunit POLR3D
**作者**：Smith A, et al.
**摘要**：该研究解析了重组人POLR3D蛋白的晶体结构，揭示了其与POLR3B亚基的关键相互作用位点，阐明了其在RNA聚合酶III复合体组装中的核心作用。
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2. **文献名称**：POLR3D dysfunction disrupts tRNA synthesis and cellular homeostasis
**作者**：Chen L, et al.
**摘要**：通过重组POLR3D蛋白功能缺失实验，发现其缺陷导致tRNA转录水平下降，引发细胞应激反应，提示POLR3D在维持细胞稳态中的重要性。
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3. **文献名称**：Recombinant POLR3D as a biomarker in cancer prognosis
**作者**：Wang X, et al.
**摘要**：研究利用重组POLR3D蛋白检测癌症患者血清中的抗体水平，发现其表达异常与结直肠癌进展相关，可能成为潜在预后标志物。
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(注：上述文献及内容为示例，实际引用需查询真实数据库如PubMed或Google Scholar。)
**Background of Recombinant Human POLR3D Protein**
POLR3D (RNA Polymerase III Subunit D), also known as RPC4. is a critical component of RNA polymerase III (Pol III), a multi-subunit enzyme responsible for transcribing small non-coding RNAs, including transfer RNAs (tRNAs), 5S ribosomal RNA (rRNA), and other regulatory RNAs essential for cellular functions like translation and RNA processing. As part of the Pol III core complex, POLR3D forms a heterodimer with POLR3K (RPC5), stabilizing the enzyme’s structure and facilitating its catalytic activity.
Recombinant human POLR3D protein is produced using genetic engineering techniques, enabling precise study of its molecular interactions and role in transcription regulation. Research highlights its involvement in maintaining cellular homeostasis, with dysregulation linked to diseases such as neurodegenerative disorders, cancer, and viral infections. Mutations in POLR3D have been associated with severe developmental conditions, including hypomyelinating leukodystrophy and cerebellar degeneration, underscoring its importance in neural health.
Recent studies focus on its potential as a therapeutic target, particularly in cancers where Pol III activity is upregulated. Recombinant POLR3D is instrumental in structural and functional analyses, aiding drug discovery and mechanistic insights into Pol III-mediated transcription. Its recombinant form also supports investigations into gene expression regulation and pathogen-host interactions, making it a valuable tool in both basic and applied biomedical research.
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