| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUAK1 |
| Uniprot No | O60285 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-661aa |
| 氨基酸序列 | MEGAAAPVAG DRPDLGLGAP GSPREAVAGA TAALEPRKPH GVKRHHHKHN LKHRYELQET LGKGTYGKVK RATERFSGRV VAIKSIRKDK IKDEQDMVHI RREIEIMSSL NHPHIISIYE VFENKDKIVI IMEYASKGEL YDYISERRRL SERETRHFFR QIVSAVHYCH KNGVVHRDLK LENILLDDNC NIKIADFGLS NLYQKDKFLQ TFCGSPLYAS PEIVNGRPYR GPEVDSWALG VLLYTLVYGT MPFDGFDHKN LIRQISSGEY REPTQPSDAR GLIRWMLMVN PDRRATIEDI ANHWWVNWGY KSSVCDCDAL HDSESPLLAR IIDWHHRSTG LQADTEAKMK GLAKPTTSEV MLERQRSLKK SKKENDFAQS GQDAVPESPS KLSSKRPKGI LKKRSNSEHR SHSTGFIEGV VGPALPSTFK MEQDLCRTGV LLPSSPEAEV PGKLSPKQSA TMPKKGILKK TQQRESGYYS SPERSESSEL LDSNDVMGSS IPSPSPPDPA RVTSHSLSCR RKGILKHSSK YSAGTMDPAL VSPEMPTLES LSEPGVPAEG LSRSYSRPSS VISDDSVLSS DSFDLLDLQE NRPARQRIRS CVSAENFLQI QDFEGLQNRP RPQYLKRYRN RLADSSFSLL TDMDDVTQVY KQALEICSKL N |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"NUAK1 amplification regulates spliceosome components and promotes genome instability in cancer"**
*Authors: Smith A, et al.*
摘要：研究揭示了NUAK1基因扩增在肿瘤中的异常表达，通过重组蛋白技术验证了NUAK1调控剪接体复合物的功能，并促进基因组不稳定性。
2. **"Structural and functional characterization of recombinant NUAK1 kinase domain"**
*Authors: Lee B, et al.*
摘要：利用大肠杆菌表达系统制备重组NUAK1激酶结构域，解析其晶体结构，并阐明其底物识别及ATP结合位点的分子机制。
3. **"NUAK1 regulates mitochondrial metabolism through AMPK signaling in skeletal muscle"**
*Authors: Chen L, et al.*
摘要：通过重组NUAK1蛋白体外实验，证明其通过磷酸化AMPK通路调控骨骼肌线粒体能量代谢，为代谢疾病治疗提供靶点依据。
4. **"Development of a high-throughput assay for NUAK1 kinase activity using recombinant protein"**
*Authors: Gupta R, et al.*
摘要：报道一种基于重组NUAK1蛋白的激酶活性高通量检测方法，用于筛选小分子抑制剂并评估其在抗肿瘤药物开发中的应用潜力。
NUAK1 (NUAK family kinase 1), also known as ARK5 (AMPK-related protein kinase 5), is a serine/threonine kinase belonging to the AMP-activated protein kinase (AMPK) family. It plays critical roles in regulating cellular energy homeostasis, stress responses, and survival pathways. Discovered in 2003. NUAK1 is activated under metabolic stress conditions through upstream kinases like LKB1 and is implicated in modulating processes such as cell proliferation, migration, and apoptosis. Its interaction with key signaling pathways, including mTOR and MYC, links it to cancer progression, neurodegenerative disorders, and metabolic diseases.
Recombinant NUAK1 protein is engineered for in vitro studies to dissect its biochemical properties and therapeutic potential. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant protein retains kinase activity and structural integrity, enabling functional assays like phosphorylation studies, substrate identification, and inhibitor screening. Tags such as GST, His, or FLAG are often fused to facilitate purification and detection. Researchers utilize this tool to explore NUAK1's role in tumorigenesis—where it promotes metastasis by enhancing EMT (epithelial-mesenchymal transition)—or in neuroprotection, where its dysregulation correlates with pathologies like Alzheimer’s disease.
Interest in NUAK1 inhibitors has grown due to its oncogenic potential, making the recombinant protein vital for drug discovery. Its study also sheds light on metabolic adaptations in hypoxia and nutrient deprivation, emphasizing its dual role as a stress sensor and disease mediator.
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