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Recombinant Human POLR2A Protein

  • 中文名: 重组人(POLR2A)蛋白
  • 别    名: DNA directed RNA polymerase II A; DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB
货号: PAX2000-10442
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点POLR2A
Uniprot NoP24928
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-110 aa
活性数据MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCV
分子量37.84 kDa
蛋白标签GST-tag at N-terminal
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下是关于重组人POLR2A蛋白的3-4篇参考文献(虚构示例,仅用于格式参考):

1. **文献名称**:*Structural insights into human RNA polymerase II by X-ray crystallography*

**作者**:Zhang Y, et al.

**摘要**:解析了重组人POLR2A蛋白的晶体结构,揭示了其催化核心区与DNA模板结合的分子机制,为转录起始和延伸提供结构基础。

2. **文献名称**:*POLR2A mutations impair transcriptional elongation in stem cells*

**作者**:Johnson M, et al.

**摘要**:通过重组POLR2A突变体研究,发现其羧基末端结构域(CTD)磷酸化异常导致胚胎干细胞分化障碍,强调其在转录调控中的关键作用。

3. **文献名称**:*Recombinant POLR2A expression and its interactome profiling*

**作者**:Lee S, et al.

**摘要**:开发了基于昆虫细胞系统的重组POLR2A高效表达纯化方法,并结合质谱分析鉴定了其与转录因子TFIIS等蛋白的相互作用网络。

4. **文献名称**:*POLR2A haploinsufficiency drives cancer genome instability*

**作者**:Wang H, et al.

**摘要**:研究发现POLR2A基因单倍剂量不足会破坏RNA聚合酶II复合体功能,导致癌细胞基因组不稳定,为靶向治疗提供新思路。

(注:上述文献为虚构内容,实际文献需通过数据库如PubMed/Web of Science检索。)


背景信息

POLR2A, also known as RNA polymerase II subunit A, is the largest catalytic subunit of RNA polymerase II (Pol II), the enzyme responsible for transcribing protein-coding genes into precursor messenger RNA (pre-mRNA) in eukaryotes. Encoded by the *POLR2A* gene in humans, this essential protein forms the core of the Pol II complex and plays a pivotal role in transcription initiation, elongation, and termination. Its C-terminal domain (CTD), composed of heptapeptide repeats (Tyr-Ser-Pro-Thr-Ser-Pro-Ser), undergoes dynamic phosphorylation modifications during transcription, enabling interactions with transcription factors, chromatin remodelers, and RNA-processing machinery.

Recombinant human POLR2A is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for structural and functional studies. Purified recombinant POLR2A retains enzymatic activity and is widely used to investigate transcription mechanisms, Pol II-related diseases (e.g., cancers linked to *POLR2A* haploinsufficiency), and therapeutic targeting. Studies leveraging recombinant POLR2A have clarified its role in stress responses, DNA repair, and promoter-proximal pausing. Its dysregulation is implicated in neurodegeneration, developmental disorders, and viral pathogenesis (e.g., HIV-1 transcription). Advances in cryo-EM and recombinant protein technology continue to unravel POLR2A's structural dynamics, offering insights into gene expression regulation and potential treatments for transcription-associated diseases.


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