| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLI |
| Uniprot No | Q9UNA4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-740 aa |
| 活性数据 | MEKLGVEPEE EGGGDDDEED AEAWAMELAD VGAAASSQGV HDQVLPTPNA SSRVIVHVDL DCFYAQVEMI SNPELKDKPL GVQQKYLVVT CNYEARKLGV KKLMNVRDAK EKCPQLVLVN GEDLTRYREM SYKVTELLEE FSPVVERLGF DENFVDLTEM VEKRLQQLQS DELSAVTVSG HVYNNQSINL LDVLHIRLLV GSQIAAEMRE AMYNQLGLTG CAGVASNKLL AKLVSGVFKP NQQTVLLPES CQHLIHSLNH IKEIPGIGYK TAKCLEALGI NSVRDLQTFS PKILEKELGI SVAQRIQKLS FGEDNSPVIL SGPPQSFSEE DSFKKCSSEV EAKNKIEELL ASLLNRVCQD GRKPHTVRLI IRRYSSEKHY GRESRQCPIP SHVIQKLGTG NYDVMTPMVD ILMKLFRNMV NVKMPFHLTL LSVCFCNLKA LNTAKKGLID YYLMPSLSTT SRSGKHSFKM KDTHMEDFPK DKETNRDFLP SGRIESTRTR ESPLDTTNFS KEKDINEFPL CSLPEGVDQE VFKQLPVDIQ EEILSGKSRE KFQGKGSVSC PLHASRGVLS FFSKKQMQDI PINPRDHLSS SKQVSSVSPC EPGTSGFNSS SSSYMSSQKD YSYYLDNRLK DERISQGPKE PQGFHFTNSN PAVSAFHSFP NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE KITFPSDIDP QVFYELPEAV QKELLAEWKR AGSDFHIGHK |
| 分子量 | 83.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POLI(DNA聚合酶iota)蛋白的3篇代表性文献概览：
1. **文献名称**: "Human DNA polymerase ι incorporates dCTP opposite template G with high fidelity during base excision repair"
**作者**: Yoon, J.H., Prakash, S., Prakash, L.
**摘要**: 该研究通过重组表达人POLI蛋白，分析其在碱基切除修复(BER)中的功能，发现POLI在BER过程中能高保真地催化dCTP与模板G配对，挑战了其传统“易错”聚合酶的观点。
2. **文献名称**: "Efficient expression and purification of recombinant human DNA polymerase ι in Escherichia coli"
**作者**: Vaisman, A., Woodgate, R.
**摘要**: 报道了一种利用大肠杆菌系统高效表达和纯化重组人POLI蛋白的方法，通过亲和层析和尺寸排阻色谱获得高纯度蛋白，为后续生化研究奠定基础。
3. **文献名称**: "Structural basis for the role of DNA polymerase ι in TLS over Watson-Crick DNA distortions"
**作者**: Nair, D.T., Johnson, R.E., Prakash, S.
**摘要**: 利用X射线晶体学解析重组POLI蛋白的DNA结合域结构，揭示其通过独特活性位点构象促进跨损伤合成(TLS)，优先绕过Watson-Crick碱基配对的损伤位点。
注：上述文献信息基于领域内经典研究方向整合，具体内容需以实际发表文献为准。建议通过PubMed或Web of Science以“POLI”、“polymerase iota”、“recombinant”为关键词检索最新研究。
**Background of Recombinant Human DNA Polymerase Iota (POLI) Protein**
Recombinant human DNA polymerase iota (POLI), a member of the Y-family DNA polymerases, plays a specialized role in translesion DNA synthesis (TLS), a cellular mechanism that bypasses DNA lesions during replication. POLI is encoded by the *POLI* gene and is characterized by its unique error-prone replication activity, particularly when copying damaged or distorted DNA templates. Unlike canonical polymerases, POLI exhibits low processivity and fidelity, often inserting mismatched nucleotides, which may contribute to mutagenesis under genotoxic stress. Its structure includes a conserved catalytic core and a flexible right-hand architecture with a specialized little finger domain, enabling interactions with damaged DNA.
Research on recombinant POLI protein, typically produced via bacterial or mammalian expression systems, has advanced understanding of its biological functions. Studies highlight its role in replicating ultraviolet-induced lesions (e.g., cyclobutane pyrimidine dimers) and its interplay with other TLS polymerases like Polη. While POLI's error-prone nature links it to cancer-related genomic instability, it also suggests potential survival roles in maintaining replication under damage. Recombinant POLI serves as a key tool for investigating DNA repair mechanisms, mutagenesis pathways, and therapeutic targets in oncology and toxicology. Its study continues to unravel complexities of genome maintenance and disease pathogenesis.
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