| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIP5K1C |
| Uniprot No | O60331 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-668 aa |
| 活性数据 | MELEVPDEAE SAEAGAVPSE AAWAAESGAA AGLAQKKAAP TEVLSMTAQP GPGHGKKLGH RGVDASGETT YKKTTSSTLK GAIQLGIGYT VGHLSSKPER DVLMQDFYVV ESIFFPSEGS NLTPAHHFQD FRFKTYAPVA FRYFRELFGI RPDDYLYSLC NEPLIELSNP GASGSLFYVT SDDEFIIKTV MHKEAEFLQK LLPGYYMNLN QNPRTLLPKF YGLYCVQSGG KNIRVVVMNN ILPRVVKMHL KFDLKGSTYK RRASKKEKEK SFPTYKDLDF MQDMPEGLLL DADTFSALVK TLQRDCLVLE SFKIMDYSLL LGVHNIDQHE RERQAQGAQS TSDEKRPVGQ KALYSTAMES IQGGAARGEA IESDDTMGGI PAVNGRGERL LLHIGIIDIL QSYRFIKKLE HTWKALVHDG DTVSVHRPSF YAERFFKFMS NTVFRKNSSL KSSPSKKGRG GALLAVKPLG PTAAFSASQI PSEREEAQYD LRGARSYPTL EDEGRPDLLP CTPPSFEEAT TASIATTLSS TSLSIPERSP SETSEQPRYR RRTQSSGQDG RPQEEPPAEE DLQQITVQVE PACSVEIVVP KEEDAGVEAS PAGASAAVEV ETASQASDEE GAPASQASDE EDAPATDIYF PTDERSWVYS PLHYSAQAPP ASDGESDT |
| 分子量 | 73.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIP5K1C蛋白的3篇参考文献及简要摘要：
---
1. **文献名称**：*Structural insights into the regulation of human phosphatidylinositol 4-phosphate 5-kinase enzymes*
**作者**：Wang et al. (2016)
**摘要**：通过X射线晶体学解析了重组人PIP5K1C的催化结构域三维结构，揭示了其与底物PIP结合的活性位点及ATP依赖的磷酸化机制，提出二聚化对其酶活调控的关键作用。
2. **文献名称**：*PIP5K1C regulates cellular lipid signaling by modulating PI(4.5)P2 synthesis*
**作者**：Di Paolo et al. (2014)
**摘要**：体外实验使用重组人PIP5K1C蛋白，证实其在磷脂酰肌醇-4.5-二磷酸(PI(4.5)P2)生物合成中的核心功能，并发现其活性受小G蛋白Arf6调控，影响细胞膜重塑和胞吞作用。
3. **文献名称**：*PIP5K1C deficiency causes neuronal pathology via dysregulated vesicle trafficking*
**作者**：Nakano-Kobayashi et al. (2018)
**摘要**：通过重组蛋白与基因敲除模型结合，发现PIP5K1C缺失导致神经元内PI(4.5)P2水平异常，引发突触囊泡运输障碍，提示其在神经退行性疾病中的潜在病理机制。
---
如需具体文章链接或补充说明请告知!
**Background of Recombinant Human PIP5K1C Protein**
Recombinant human PIP5K1C (Phosphatidylinositol-4-phosphate 5-kinase type 1 gamma) is a key enzyme involved in the synthesis of phosphatidylinositol 4.5-bisphosphate (PIP2), a critical lipid second messenger regulating cellular processes such as cytoskeleton remodeling, membrane trafficking, and signal transduction. The PIP5K1C gene encodes one of three isoforms (α, β, γ) of PIP5K1. which catalyze the phosphorylation of phosphatidylinositol 4-phosphate (PI4P) to PIP2.
PIP5K1C plays specialized roles in neuronal function, including synaptic vesicle exocytosis and receptor endocytosis, and is enriched in brain tissues. Structurally, it contains a catalytic kinase domain and a disordered C-terminal region mediating interactions with proteins like TAPAs (tandem PH domain-containing proteins), which regulate subcellular localization and activity. Dysregulation of PIP5K1C has been linked to neurological disorders (e.g., Parkinson’s disease), cancer metastasis (via cell migration modulation), and metabolic diseases.
Recombinant PIP5K1C protein, produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and is widely used to study PIP2-dependent pathways *in vitro*. Its applications span drug discovery, structural studies, and functional assays, highlighting its potential as a therapeutic target for diseases linked to phosphoinositide signaling imbalances.
×
