| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIP5K1B |
| Uniprot No | O14986 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-540 aa |
| 活性数据 | MSSAAENGEA APGKQNEEKT YKKTASSAIK GAIQLGIGYT VGNLTSKPER DVLMQDFYVV ESVFLPSEGS NLTPAHHYPD FRFKTYAPLA FRYFRELFGI KPDDYLYSIC SEPLIELSNP GASGSLFFVT SDDEFIIKTV QHKEAEFLQK LLPGYYMNLN QNPRTLLPKF YGLYCMQSGG INIRIVVMNN VLPRSMRMHF TYDLKGSTYK RRASRKEREK SNPTFKDLDF LQDMHEGLYF DTETYNALMK TLQRDCRVLE SFKIMDYSLL LGIHFLDHSL KEKEEETPQN VPDAKRTGMQ KVLYSTAMES IQGPGKSGDG IITENPDTMG GIPAKSHRGE KLLLFMGIID ILQSYRLMKK LEHSWKALVY DGDTVSVHRP SFYADRFLKF MNSRVFKKIQ ALKASPSKKR CNSIAALKAT SQEIVSSISQ EWKDEKRDLL TEGQSFSSLD EEALGSRHRP DLVPSTPSLF EAASLATTIS SSSLYVNEHY PHDRPTLYSN SKGLPSSSTF TLEEGTIYLT AEPNTLEVQD DNASVLDVYL |
| 分子量 | 61.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIP5K1B蛋白的参考文献示例，包括文献名称、作者及摘要概括：
1. **《Structural insights into PIP5K1B kinase activity and membrane interaction》**
- **作者**：Li, X., Zhang, Y., & Cheng, H.
- **摘要**：该研究通过X射线晶体学解析了重组人PIP5K1B蛋白的催化结构域三维结构，揭示了其与磷脂酸结合的关键位点，并阐明了其催化生成PIP2的分子机制，为靶向PIP5K1B的药物设计提供了结构基础。
2. **《PIP5K1B regulates EGFR endocytosis and signaling in cancer cells》**
- **作者**：Wang, L., et al.
- **摘要**：本文发现重组PIP5K1B通过调控细胞膜上PIP2的生成，影响表皮生长因子受体(EGFR)的内吞和下游信号通路，促进肺癌细胞的迁移和侵袭，提示其在肿瘤转移中的潜在作用。
3. **《A high-throughput assay for PIP5K1B enzymatic activity using recombinant protein》**
- **作者**：Johnson, R.S., & Patel, A.K.
- **摘要**：作者开发了一种基于荧光底物的高通量检测方法，利用重组PIP5K1B蛋白筛选激酶抑制剂，验证了多个小分子化合物对PIP5K1B活性的抑制效果，为疾病治疗提供候选分子。
4. **《PIP5K1B deficiency impairs synaptic plasticity and cognitive function in mice》**
- **作者**：Chen, M., et al.
- **摘要**：研究通过基因敲除模型发现，PIP5K1B缺失导致小鼠海马神经元突触PIP2水平下降，影响突触可塑性和学习记忆能力，表明其在神经系统功能中的关键性。
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注：以上文献信息为示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认具体作者及细节。
**Background of Recombinant Human PIP5K1B Protein**
Phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1B) is a member of the phosphatidylinositol-4-phosphate 5-kinase family, which catalyzes the synthesis of phosphatidylinositol-4.5-bisphosphate (PIP2), a critical lipid secondary messenger involved in cellular signaling, membrane trafficking, and cytoskeletal organization. PIP5K1B, encoded by the *PIP5K1B* gene in humans, is one of three isoforms (1α, 1β, 1γ) with distinct tissue distributions and regulatory roles. It localizes to the plasma membrane and endosomes, interacting with signaling proteins like small GTPases and receptor tyrosine kinases.
Structurally, PIP5K1B contains an N-terminal catalytic domain and a C-terminal regulatory region. Its activity is modulated by extracellular stimuli, including growth factors and cytokines, through pathways such as Rho GTPases and PI3K/AKT. Dysregulation of PIP5K1B is linked to pathologies like cancer (enhanced proliferation, invasion), neurodegenerative disorders (synaptic dysfunction), and metabolic diseases.
Recombinant human PIP5K1B protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables mechanistic studies of PIP2-dependent processes and drug discovery targeting PIP5K1B-associated diseases. Its purified form supports structural analysis, enzymatic assays, and inhibitor screening, aiding the development of therapies modulating PIP2 signaling. Research on PIP5K1B continues to unravel its nuanced roles in health and disease.
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