| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIP4K2C |
| Uniprot No | Q8TBX8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-421 aa |
| 活性数据 | ASSSVPPAT VSAATAGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV FLWGVAHSIN ELSQVPPPVM LLPDDFKASS KIKVNNHLFH RENLPSHFKF KEYCPQVFRN LRDRFGIDDQ DYLVSLTRNP PSESEGSDGR FLISYDRTLV IKEVSSEDIA DMHSNLSNYH QYIVKCHGNT LLPQFLGMYR VSVDNEDSYM LVMRNMFSHR LPVHRKYDLK GSLVSREASD KEKVKELPTL KDMDFLNKNQ KVYIGEEEKK IFLEKLKRDV EFLVQLKIMD YSLLLGIHDI IRGSEPEEEA PVREDESEVD GDCSLTGPPA LVGSYGTSPE GIGGYIHSHR PLGPGEFESF IDVYAIRSAE GAPQKEVYFM GLIDILTQYD AKKKAAHAAK TVKHGAGAEI STVHPEQYAK RFLDFITNIF A |
| 分子量 | 47.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIP4K2C蛋白的3篇参考文献示例(注：内容为模拟虚构，仅供参考)：
1. **文献名称**：*Structural Insights into PIP4K2C Regulation by Small Molecule Inhibitors*
**作者**：Thompson, R. et al.
**摘要**：通过X射线晶体学解析了重组人PIP4K2C蛋白的三维结构，揭示了其与ATP和磷酸肌醇结合的活性位点，并筛选出特异性抑制剂在肿瘤细胞中调控PI3K/AKT通路的作用机制。
2. **文献名称**：*PIP4K2C Recombinant Protein Expression and Functional Characterization in Neurodegenerative Models*
**作者**：Chen, L. & Wang, H.
**摘要**：利用杆状病毒-昆虫细胞系统高效表达重组人PIP4K2C蛋白，证实其磷酸化PI(5)P生成PI(4.5)P2的功能，并发现其在阿尔茨海默病模型中调节神经元内囊泡运输的异常。
3. **文献名称**：*CRISPR/Cas9-Mediated PIP4K2C Knockout Combined with Recombinant Protein Rescue Validates Its Role in Autophagy*
**作者**：Garcia, M. et al.
**摘要**：通过基因编辑技术构建PIP4K2C缺陷细胞系，利用重组PIP4K2C蛋白回补实验证明其在自噬启动中的必要性，揭示了PIP4K2C通过调控mTORC1信号通路影响肿瘤细胞能量代谢。
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*注：以上文献为生成模拟内容，实际引用需检索真实数据库(如PubMed、Web of Science)并核实原文。*
PIP4K2C (Phosphatidylinositol-5-Phosphate 4-Kinase Type 2 Gamma) is a member of the PIP4K family, enzymes that phosphorylate phosphatidylinositol-5-phosphate (PIP5) to generate phosphatidylinositol-4.5-bisphosphate (PIP2), a critical lipid secondary messenger regulating cellular signaling, membrane trafficking, and cytoskeletal dynamics. Unlike other PIP4K isoforms (PIP4K2A/B), PIP4K2C exhibits distinct subcellular localization and functional roles, often associating with membranes and organelles such as lysosomes. It plays a role in modulating autophagy, lysosomal function, and cellular stress responses, with implications in cancer, neurodegenerative diseases, and immune regulation. Structurally, PIP4K2C contains a conserved kinase domain and unique N-terminal regions that influence its interaction partners and activity. Dysregulation of PIP4K2C has been linked to tumor progression, drug resistance, and metabolic disorders, making it a potential therapeutic target. Recombinant human PIP4K2C protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used in biochemical assays, structural studies, and inhibitor screening to elucidate its mechanistic roles and develop targeted therapies. Recent studies also highlight its involvement in T-cell signaling and neurodegenerative pathways, underscoring its multifaceted biological significance.
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