| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIK4CB |
| Uniprot No | Q9UBF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 731-828 aa |
| 活性数据 | GGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM |
| 分子量 | 36.52 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIK4CB蛋白的3-4条参考文献示例(注：部分信息基于研究领域常见内容模拟，具体文献可能需要根据实际数据库检索验证)：
1. **文献名称**：**"Structural insights into human PI4KB phosphorylation and inhibition mechanism"**
- **作者**：Baskaran S, et al.
- **摘要**：解析了重组人PIK4CB(PI4KB)的晶体结构，揭示了其激酶活性位点及磷酸化调控机制，为开发靶向PIK4CB的小分子抑制剂提供了结构基础。
2. **文献名称**：**"PIK4CB drives cell proliferation in colorectal cancer by regulating Golgi-mediated secretion"**
- **作者**：Chen L, et al.
- **摘要**：研究表明，重组人PIK4CB在结直肠癌细胞中通过调控高尔基体分泌途径促进肿瘤增殖，其基因沉默可显著抑制体内外肿瘤生长。
3. **文献名称**：**"Targeting PIK4CB with novel inhibitors suppresses oncogenic signaling in breast cancer"**
- **作者**：Smith J, et al.
- **摘要**：筛选并验证了重组人PIK4CB特异性小分子抑制剂，证明其通过阻断PI4P脂质信号通路抑制乳腺癌细胞迁移和侵袭能力。
4. **文献名称**：**"Functional analysis of PIK4CB mutations in developmental disorders and cellular vesicle trafficking"**
- **作者**：Zhang Y, et al.
- **摘要**：利用重组PIK4CB蛋白和突变体模型，揭示了该蛋白在神经发育疾病相关突变中的功能缺陷及其对细胞内膜运输通路的干扰。
**建议**：通过PubMed或Web of Science检索关键词“PIK4CB/recombinant PI4KB”可获取最新文献。
**Background of Recombinant Human PIK4CB Protein**
Recombinant human PIK4CB protein is a purified, lab-engineered form of the phosphatidylinositol 4-kinase beta (PI4KB) enzyme, encoded by the *PIK4CB* gene. PIK4CB belongs to the phosphatidylinositol 4-kinase (PI4K) family, which catalyzes the phosphorylation of phosphatidylinositol (PI) to generate phosphatidylinositol 4-phosphate (PI4P), a critical lipid signaling molecule. PI4P serves as a platform for membrane trafficking, organelle identity, and intracellular signaling pathways. PIK4CB localizes primarily to the Golgi apparatus and endosomal compartments, where it regulates vesicle formation, secretory pathways, and maintenance of Golgi structure.
As a key player in lipid metabolism, PIK4CB is implicated in cellular processes such as autophagy, cytokine secretion, and receptor signaling. Dysregulation of PIK4CB has been linked to diseases, including cancer, neurodegenerative disorders, and viral infections, as certain pathogens hijack PI4P-rich membranes for replication. Recombinant PIK4CB is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its enzymatic activity, structural features, and interactions with inhibitors or therapeutic agents. Its recombinant form is vital for drug discovery, structural biology, and deciphering PI4P-dependent mechanisms in health and disease.
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