| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIK3R6 |
| Uniprot No | Q5UE93 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-754 aa |
| 活性数据 | MESSDVELDL QRSVQAVLRE LSTQAPALQS NQGMWRWSLH KKVERDPGKS PVLVRILLRE LEKAESQDLR HVIIPLLHTV MYVLTKATGI TEELYQRIYA FCTRLLTLPT PYCTVALDCA IRLKTEMAVP GTLYQRMVIA EQNLTNELYP YQERVFLFVD PELVSASVCS ALLLEIEAAQ AQQTPETCMR HVVSHALQAA LGEACHAGAL HRKLQASPRR TLEHYFHAVV AALEQMASEA SPSREGHVER LEEIYCSLLG PAAGRCGGDL VQERPPSIPL PSPYITFHLW TGEEQLWKEL VLFLRPRSQL RLSADLEVLD LQGLRPDREL ARVSVLSTDS GIERDLPTGA DELPAPGSPE MERAGLQRKG GIKKRAWPLD FLMPGSWDGP PGLHRRTGRP SGDGEMLPGV SRLHTARVLV LGDDRMLGRL AQAYHRLRKR ETQKFCLTPR LSLQLYYIPV LAPEKPAASR QPELGELATF LGRVDPWYQS NVNTLCPAIH KLAEMPPSLD TSRTVDPFIL DVITYYIRMG TQPIYFQIYT VKIFFSDLSQ DPTEDIFLIE LKVKIQDSKF PKDGFSPRRR GVAEGPGAEL SLCYQKALLS HRPREVTVSL RATGLILKAI PASDTEVSGS SHCPLPAAPV TDHTCLNVNV TEVVKSSNLA GKSFSTVTNT FRTNNIQIQS RDQRLLTLSL DKDDQRTFRD VVRFEVAPCP EPCSGAQKSK APWLNLHGQQ EVEAIKAKPK PLLMPINTFS GIVQ |
| 分子量 | 84.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIK3R6蛋白的参考文献示例(请注意，部分文献为假设性概括，实际引用时请核实文献真实性和具体内容)：
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1. **文献名称**：*"Efficient Expression and Functional Characterization of Recombinant Human PIK3R6 in Mammalian Cells"*
**作者**：Lee S, et al.
**摘要**：本研究报道了在HEK293细胞中高效表达重组人PIK3R6蛋白的方法，利用亲和层析技术纯化蛋白并验证其与PI3K催化亚基(p110γ)的结合能力。结果显示，重组PIK3R6显著增强PI3K酶活性，为其在信号转导中的调控机制提供了实验依据。
2. **文献名称**：*"Structural Basis of PIK3R6-mediated Regulation of PI3K Lipid Kinase Activity"*
**作者**：Wang Y, et al.
**摘要**：通过冷冻电镜技术解析了重组人PIK3R6与p110γ/p84复合物的三维结构，揭示了PIK3R6通过特定结构域抑制PI3K活性的分子机制，为靶向PI3K信号通路的药物设计提供了结构学基础。
3. **文献名称**：*"Role of Recombinant PIK3R6 in Immune Cell Signaling and Inflammatory Response"*
**作者**：Garcia M, et al.
**摘要**：利用重组PIK3R6蛋白处理巨噬细胞，发现其通过调控PI3K-Akt-mTOR通路抑制炎症因子释放，表明PIK3R6在免疫调节中可能具有潜在治疗价值。
4. **文献名称**：*"Development of a PIK3R6 Knockout Model and Rescue with Recombinant Protein in Cancer Cells"*
**作者**：Tanaka R, et al.
**摘要**：通过CRISPR技术构建PIK3R6基因敲除的癌细胞系，发现敲除导致PI3K信号过度激活。外源性添加重组PIK3R6蛋白可逆转该效应，证实其在维持信号平衡中的关键作用。
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**注意事项**：
- 上述文献及摘要内容为示例性质，实际研究中可能需根据具体研究方向检索真实文献(建议使用PubMed、Google Scholar等平台)。
- 若针对性文献较少，可扩展检索范围至**PI3K调节亚基家族**(如PIK3R1/PIK3R3)或相关通路的研究，并结合关键词“recombinant protein production”或“signal transduction”筛选。
**Background of Recombinant Human PIK3R6 Protein**
Phosphoinositide-3-kinase regulatory subunit 6 (PIK3R6) is a regulatory subunit of the class III phosphatidylinositol 3-kinase (PI3K) complex, which plays a critical role in intracellular membrane trafficking, autophagy, and endosomal sorting. Unlike other PI3K classes involved in cell growth and survival signaling, class III PI3K, including PIK3R6. primarily regulates vesicle-mediated transport and lysosomal functions. PIK3R6 interacts with the catalytic subunit VPS34 (PIK3C3) to form the core complex, stabilizing its enzymatic activity and directing lipid kinase activity toward generating phosphatidylinositol-3-phosphate (PI3P). This lipid second messenger recruits effector proteins containing FYVE or PX domains to coordinate membrane remodeling and trafficking processes.
Recombinant human PIK3R6 protein is engineered for research to study its regulatory mechanisms, protein interactions, and its role in diseases linked to autophagy dysregulation, such as cancer and neurodegenerative disorders. Produced typically in *E. coli* or mammalian expression systems, the recombinant protein retains functional domains necessary for binding VPS34 and modulating PI3P production. Its application spans *in vitro* assays, structural studies, and drug discovery, particularly for therapeutics targeting autophagy-dependent pathways. Understanding PIK3R6's function provides insights into cellular homeostasis and potential interventions for pathologies involving vesicle transport defects.
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