| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIK3R1 |
| Uniprot No | P27986 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-724 aa |
| 活性数据 | SAEGYQYRA LYDYKKEREE DIDLHLGDIL TVNKGSLVAL GFSDGQEARP EEIGWLNGYN ETTGERGDFP GTYVEYIGRK KISPPTPKPR PPRPLPVAPG SSKTEADVEQ QALTLPDLAE QFAPPDIAPP LLIKLVEAIE KKGLECSTLY RTQSSSNLAE LRQLLDCDTP SVDLEMIDVH VLADAFKRYL LDLPNPVIPA AVYSEMISLA PEVQSSEEYI QLLKKLIRSP SIPHQYWLTL QYLLKHFFKL SQTSSKNLLN ARVLSEIFSP MLFRFSAASS DNTENLIKVI EILISTEWNE RQPAPALPPK PPKPTTVANN GMNNNMSLQD AEWYWGDISR EEVNEKLRDT ADGTFLVRDA STKMHGDYTL TLRKGGNNKL IKIFHRDGKY GFSDPLTFSS VVELINHYRN ESLAQYNPKL DVKLLYPVSK YQQDQVVKED NIEAVGKKLH EYNTQFQEKS REYDRLYEEY TRTSQEIQMK RTAIEAFNET IKIFEEQCQT QERYSKEYIE KFKREGNEKE IQRIMHNYDK LKSRISEIID SRRRLEEDLK KQAAEYREID KRMNSIKPDL IQLRKTRDQY LMWLTQKGVR QKKLNEWLGN ENTEDQYSLV EDDEDLPHHD EKTWNVGSSN RNKAENLLRG KRDGTFLVRE SSKQGCYACS VVVDGEVKHC VINKTATGYG FAEPYNLYSS LKELVLHYQH TSLVQHNDSL NVTLAYPVYA QQRR |
| 分子量 | 83.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIK3R1蛋白的3篇参考文献示例(简化的标题、作者和摘要内容)：
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1. **标题**：*Structural insights into the regulation of PI3Kα by the p85α subunit*
**作者**：Huang, C.H., et al.
**摘要**：本研究通过X射线晶体学解析了p85α(PIK3R1编码)与PI3Kα催化亚基的复合物结构，揭示了p85α通过SH2和iSH2结构域抑制激酶活性的分子机制，为靶向PI3K信号通路的药物设计提供依据。
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2. **标题**：*Mutations in PIK3R1 disrupt phosphoinositide metabolism and cause immune dysregulation*
**作者**：Lucas, C.L., et al.
**摘要**：研究发现PIK3R1功能缺失性突变会导致免疫缺陷综合征，重组p85α蛋白实验表明突变体无法正常结合PI3K催化亚基，进而干扰PI3K-AKT信号传导，导致T细胞发育异常。
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3. **标题**：*Recombinant p85α protein binds to activated IRS-1 and modulates insulin signaling*
**作者**：Taniguchi, C.M., et al.
**摘要**：利用重组人p85α蛋白进行体外结合实验，证实其通过SH2结构域识别磷酸化的IRS-1.调节胰岛素信号通路的反馈抑制，为代谢疾病中PI3K通路异常提供机制解释。
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**注意**：以上文献信息为模拟示例，实际引用请以真实发表的论文为准。建议通过PubMed或Web of Science以关键词“PIK3R1”、“p85α”或“recombinant PI3K regulatory subunit”检索最新研究。
Phosphoinositide 3-kinase regulatory subunit 1 (PIK3R1), encoding the p85α regulatory subunit of class IA PI3K, plays a central role in signal transduction pathways regulating cell proliferation, survival, metabolism, and apoptosis. As a key component of the PI3K complex, p85α binds to receptor tyrosine kinases via its SH2 domains, enabling activation of the catalytic p110 subunit and subsequent generation of phosphatidylinositol (3.4.5)-trisphosphate (PIP3). This lipid messenger recruits downstream effectors such as AKT and mTOR, driving critical cellular processes.
Mutations in PIK3R1 are implicated in various diseases. Germline variants are associated with immune dysregulation syndromes (e.g., APDS2) and insulin resistance, while somatic mutations contribute to cancer progression through constitutive PI3K pathway activation. Structural studies reveal that p85α contains multiple functional domains, including SH3. RhoGAP, and proline-rich regions, enabling protein-protein interactions and regulatory cross-talk.
Recombinant human PIK3R1 protein is widely used to investigate PI3K-AKT pathway mechanisms, drug-target interactions, and mutation impacts. Produced in bacterial or mammalian expression systems, it retains binding capacity for partner proteins and serves as a critical tool for biochemical assays, structural analysis, and therapeutic discovery. Research on recombinant p85α continues to advance precision oncology and our understanding of metabolic disorders linked to PI3K dysregulation.
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