| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIGY |
| Uniprot No | Q3MUY2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-71 aa |
| 活性数据 | MFLSLPTLTVLIPLVSLAGLFYSASVEENFPQGCTSTASLCFYSLLLPITIPVYVFFHLWTWMGIKLFRHN |
| 分子量 | 34.21 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIGY蛋白的参考文献示例(注：部分内容为假设性概括，实际文献需通过学术数据库进一步确认)：
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1. **标题**：*Recombinant Human PIGY Protein Expression and Its Role in GPI Biosynthesis*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组人PIGY蛋白，证实其作为GPI转酰胺酶复合物的亚基，对细胞表面GPI锚定蛋白的合成具有关键催化功能。
2. **标题**：*Structural and Functional Analysis of the PIGY Subunit in the GPI Transamidase Complex*
**作者**：Hirota Y, et al.
**摘要**：利用哺乳动物细胞系统重组表达PIGY，结合CRISPR/Cas9技术，揭示PIGY与PIGK、PIGS的相互作用机制，阐明其在GPI锚定前体蛋白加工中的结构重要性。
3. **标题**：*Defective GPI Anchor Synthesis Due to PIGY Mutations: Recombinant Protein Rescue in Patient Cells*
**作者**：Wang L, et al.
**摘要**：在GPI缺陷型细胞中表达重组人PIGY蛋白，证明其可恢复GPI锚定蛋白功能，为遗传性糖基化疾病(如PGM3-CDG)提供分子机制证据。
4. **标题**：*Reconstitution of GPI Transamidase Activity Using Recombinant PIGY and Partner Proteins*
**作者**：Kinoshita T, et al.
**摘要**：通过共表达PIGY、PIGK等重组蛋白，在体外重建功能性GPI转酰胺酶复合物，验证其催化GPI锚定连接的生化活性。
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**注意**：以上为基于领域知识的示例，实际文献需查阅PubMed、Google Scholar等平台(可检索关键词：PIGY, GPI transamidase, recombinant expression)。真实文献可能集中于PIGY的基因功能或复合物研究，直接涉及重组蛋白制备的公开资料较少。
The PIGY protein, also known as phosphatidylinositol glycan anchor biosynthesis class Y, is a critical component in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. GPI anchors are glycolipid structures that tether specific proteins to cell membranes, enabling their localization and functional regulation. PIGY functions as a subunit of the GPI transamidase complex, which catalyzes the attachment of preassembled GPI anchors to precursor proteins in the endoplasmic reticulum. This post-translational modification is essential for the membrane association of numerous eukaryotic proteins involved in immune response, cell signaling, and adhesion.
Mutations in the PIGY gene are linked to inherited GPI deficiency disorders, such as paroxysmal nocturnal hemoglobinuria (PNH), where defective GPI anchoring leads to complement-mediated hemolysis. Recombinant human PIGY protein, produced via expression systems like mammalian cells or E. coli, serves as a vital tool for studying GPI biosynthesis mechanisms and related pathologies. Researchers employ it to reconstitute GPI-anchored proteins in vitro, investigate enzymatic interactions within the transamidase complex, and screen therapeutic agents targeting GPI-associated diseases. Its recombinant form also aids in elucidating PIGY's structural motifs and functional domains, advancing precision medicine approaches for rare genetic disorders. Current studies explore its role in cancer progression and neurological conditions, emphasizing its broad biomedical relevance.
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