| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEX16 |
| Uniprot No | Q9Y5Y5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346 aa |
| 活性数据 | MEKLRLLGLRYQEYVTRHPAATAQLETAVRGFSYLLAGRFADSHELSELVYSASNLLVLLNDGILRKELRKKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGELGRWLVIALIQLAKAVLRILLLLWFKAGLQTSPPIAPLDRETQAQPPDGDHSPGNHEQSYVGKRSNRVVRTLQNTPSLHSRHWGAPQQREGRQQQHHEELSATPTPLGLQETIAEFLYIARPLLHLLSLGLWGQRSWKPWLLAGVVDVTSLSLLSDRKGLTRRERRELRRRTILLLYYLLRSPFYDRFSEARILFLLQLLADHVPGVGLVTTSQRAASPCLPARPHTQPWSPPAFLPGHP |
| 分子量 | 63.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PEX16蛋白的**示例参考文献**(格式为模拟概括，实际文献请通过学术数据库查询)：
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1. **文献名称**：*PEX16 is required for the formation of peroxisomal membrane structures in mammalian cells*
**作者**：Honsho, M., et al.
**摘要**：研究揭示了PEX16在哺乳动物细胞过氧化物酶体膜组装中的关键作用，通过基因敲除实验表明其缺失导致膜结构异常，影响过氧化物酶体功能。
2. **文献名称**：*Mutations in PEX16 underlie Zellweger syndrome cases with impaired peroxisome biogenesis*
**作者**：Ebberink, M.S., et al.
**摘要**：报道PEX16基因突变与齐薇格综合征(Zellweger syndrome)的关联，发现患者成纤维细胞中PEX16功能缺陷导致过氧化物酶体无法正常生成。
3. **文献名称**：*PEX16 interacts with PEX19 and mediates the targeting of membrane proteins to peroxisomes*
**作者**：Matsuzaki, T., Fujiki, Y.
**摘要**：阐明PEX16与伴侣蛋白PEX19的相互作用机制，提出PEX16作为膜受体协助其他过氧化物酶体膜蛋白定位的分子机制。
4. **文献名称**：*Functional characterization of human PEX16 in yeast reveals conserved roles in peroxisome membrane formation*
**作者**：Lanyon-Hogg, T., et al.
**摘要**：利用酵母模型验证人类PEX16的功能保守性，证明其能恢复酵母突变体的过氧化物酶体缺陷，证实其在膜形成中的进化保守作用。
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**备注**：以上为基于PEX16研究领域的典型方向模拟的文献示例，实际文献需通过**PubMed/Google Scholar**以“PEX16”、“peroxisome biogenesis”等关键词检索。近年研究可能更侧重其结构解析、疾病突变谱或治疗探索。
PEX16 (Peroxisomal Biogenesis Factor 16) is a critical protein involved in the formation and maintenance of peroxisomes, membrane-bound organelles essential for lipid metabolism, detoxification, and redox balance. It functions as a peroxisomal membrane protein required for the early stages of peroxisome biogenesis, acting as a docking site for other peroxins (PEX proteins) and facilitating the recruitment of lipids and membrane proteins. PEX16 is unique in its dual localization, found both in the endoplasmic reticulum (ER) and peroxisomal membranes, suggesting a role in ER-derived vesicular trafficking for peroxisome assembly.
Mutations in the *PEX16* gene are linked to severe peroxisome biogenesis disorders (PBDs), such as Zellweger spectrum disorders, characterized by neurological dysfunction, hepatic abnormalities, and developmental delays. These autosomal recessive disorders stem from impaired peroxisomal protein import and disrupted lipid metabolism.
Recombinant human PEX16 protein is produced via genetic engineering in heterologous systems (e.g., *E. coli* or mammalian cells) for functional studies. Its recombinant form enables detailed structural analysis, interaction mapping with other peroxins, and investigation of pathogenic mechanisms in PBDs. Researchers also utilize it to explore therapeutic strategies, including gene therapy or protein replacement, aiming to restore peroxisomal function in affected cells. Ongoing studies focus on elucidating its molecular interactions and regulatory networks in peroxisome dynamics.
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