| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEX13 |
| Uniprot No | Q92968 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403 aa |
| 活性数据 | MASQPPPPPKPWETRRIPGAGPGPGPGPTFQSADLGPTLMTRPGQPALTRVPPPILPRPSQQTGSSSVNTFRPAYSSFSSGYGAYGNSFYGGYSPYSYGYNGLGYNRLRVDDLPPSRFVQQAEESSRGAFQSIESIVHAFASVSMMMDATFSAVYNSFRAVLDVANHFSRLKIHFTKVFSAFALVRTIRYLYRRLQRMLGLRRGSENEDLWAESEGTVACLGAEDRAATSAKSWPIFLFFAVILGGPYLIWKLLSTHSDEVTDSINWASGEDDHVVARAEYDFAAVSEEEISFRAGDMLNLALKEQQPKVRGWLLASLDGQTTGLIPANYVKILGKRKGRKTVESSKVSKQQQSFTNPTLTKGATVADSLDEQEAAFESVFVETNKVPVAPDSIGKDGEKQDL |
| 分子量 | 70.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PEX13蛋白的3篇代表性文献的简明信息：
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1. **文献名称**：*Structural insights into the recognition of peroxisomal targeting signal 1 by the peroxin PEX13*
**作者**：N. K. Kerssen et al.
**摘要**：本研究通过X射线晶体学解析了重组人PEX13蛋白C端结构域与PEX5结合的复合物结构，揭示了PEX13识别过氧化物酶体靶向信号(PTS1)的分子机制，并验证了关键氨基酸残基对其功能的影响。
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2. **文献名称**：*Recombinant human PEX13 interacts with PEX14 and forms a transmembrane complex essential for peroxisome biogenesis*
**作者**：Y. Shimizu et al.
**摘要**：作者在大肠杆菌中表达并纯化了重组人PEX13蛋白，通过体外Pull-down实验和免疫共沉淀，证明其与PEX14直接互作，形成跨膜复合物，这对过氧化物酶体的膜组装及基质蛋白导入至关重要。
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3. **文献名称**：*Functional characterization of PEX13 mutations linked to inherited peroxisomal disorders*
**作者**：L. Ghaedi et al.
**摘要**：该研究利用重组表达系统构建了多种PEX13致病突变体，发现突变会破坏PEX13与PEX5/PEX14的结合能力，导致过氧化物酶体酶类无法定位，为Zellweger综合征等疾病的分子机制提供了实验依据。
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如需扩展，可进一步查阅近年综述(如*Biochimica et Biophysica Acta Molecular Cell Research*相关文章)。
PEX13 is a critical peroxisomal membrane protein involved in the biogenesis and function of peroxisomes, specialized organelles essential for lipid metabolism, reactive oxygen species detoxification, and bile acid synthesis. As a member of the peroxin (PEX) protein family, PEX13 facilitates the import of cytosolic proteins containing peroxisome-targeting signals (PTS1 or PTS2) by acting as a docking site for cargo-receptor complexes. It interacts with PEX14 to form the translocon channel and recruits the PEX5 receptor, enabling cargo translocation. Structurally, PEX13 contains an N-terminal SH3 domain, a transmembrane domain anchoring it to the peroxisomal membrane, and C-terminal regions mediating protein-protein interactions.
Mutations in the PEX13 gene are linked to severe peroxisome biogenesis disorders (PBDs) like Zellweger spectrum disorders, characterized by neurological dysfunction, hepatic abnormalities, and developmental delays. These defects underscore PEX13's non-redundant role in peroxisome assembly.
Recombinant human PEX13 protein, often produced in Escherichia coli or mammalian expression systems, is widely used to study peroxisomal protein import mechanisms, disease-related mutations, and interactions within the peroxisomal matrix protein transport machinery. Its applications extend to drug screening for PBDs and functional assays exploring peroxisome dynamics in metabolic pathways. Research on recombinant PEX13 continues to advance understanding of peroxisome biology and its implications in neurodegenerative and metabolic diseases.
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