| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PEX10 |
| Uniprot No | O60683 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326 aa |
| 活性数据 | MAPAAASPPE VIRAAQKDEY YRGGLRSAAG GALHSLAGAR KWLEWRKEVE LLSDVAYFGL TTLAGYQTLG EEYVSIIQVD PSRIHVPSSL RRGVLVTLHA VLPYLLDKAL LPLEQELQAD PDSGRPLQGS LGPGGRGCSG ARRWMRHHTA TLTEQQRRAL LRAVFVLRQG LACLQRLHVA WFYIHGVFYH LAKRLTGITY LRVRSLPGED LRARVSYRLL GVISLLHLVL SMGLQLYGFR QRQRARKEWR LHRGLSHRRA SLEERAVSRN PLCTLCLEER RHPTATPCGH LFCWECITAW CSSKAECPLC REKFPPQKLI YLRHYR |
| 分子量 | 37.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PEX10蛋白的3-4篇参考文献示例(注：以下为模拟生成的文献，实际引用请通过学术数据库核实)：
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1. **文献名称**: *Molecular Cloning and Functional Characterization of Human PEX10*
**作者**: Braverman, N., et al.
**摘要**: 本研究克隆了人PEX10基因，并在哺乳动物细胞中表达重组PEX10蛋白，证实其参与过氧化物酶体膜蛋白的靶向过程。通过体外实验发现，PEX10的缺陷导致过氧化物酶体酶导入障碍，提示其在细胞器生物合成中的关键作用。
2. **文献名称**: *Domain Analysis of PEX10 Reveals Its Role in Peroxisomal Matrix Protein Import*
**作者**: Tamura, S., et al.
**摘要**: 通过重组表达PEX10的不同结构域(如RING指结构域和跨膜区域)，发现RING结构域对PEX10与其他PEX蛋白(如PEX2和PEX12)的相互作用至关重要，且其酶活性影响过氧化物酶体基质蛋白的输入效率。
3. **文献名称**: *PEX10 Mutations Cause Zellweger Syndrome by Disrupting Peroxisome Membrane Assembly*
**作者**: Waterham, H.R., et al.
**摘要**: 研究分析了齐薇格综合征患者的PEX10突变体，利用重组突变蛋白揭示其导致过氧化物酶体膜无法正常形成，并通过酵母互补实验证实突变位点与疾病表型的直接关联。
4. **文献名称**: *Interaction of Recombinant PEX10 with PEX5 and PEX19 in Peroxisome Biogenesis*
**作者**: Matsuzono, Y., et al.
**摘要**: 通过体外重组实验和免疫共沉淀技术，证明重组人PEX10蛋白与胞质受体PEX5及膜锚定因子PEX19存在直接相互作用，为PEX10在过氧化物酶体膜蛋白组装中的功能提供了机制支持。
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建议通过PubMed、Google Scholar等平台，使用关键词“recombinant human PEX10”、“PEX10 peroxisome”等获取最新文献。
Recombinant human PEX10 protein is a genetically engineered form of the peroxisomal biogenesis factor 10. a critical component in the assembly and function of peroxisomes—membrane-bound organelles essential for lipid metabolism, reactive oxygen species detoxification, and biosynthesis of plasmalogens. The PEX10 gene encodes a 44 kDa protein containing a zinc-binding RING finger domain, which facilitates its role in peroxisomal membrane protein import and ubiquitination processes. Mutations in PEX10 are linked to peroxisome biogenesis disorders (PBDs) such as Zellweger syndrome, a severe autosomal recessive condition characterized by neurological dysfunction, hepatic abnormalities, and early mortality.
Recombinant PEX10 is typically produced in *E. coli* or mammalian expression systems, enabling studies on peroxisome dynamics, protein-protein interactions, and mechanisms underlying PBDs. Its applications span structural biology, drug screening for peroxisomal disorders, and functional complementation assays to validate pathogenic variants. Research using recombinant PEX10 has advanced understanding of peroxisomal matrix protein import pathways and potential therapeutic strategies, including gene therapy or small-molecule correctors. The protein’s availability also supports diagnostic tool development for PBDs, aiding in mutation classification and personalized medicine approaches. Overall, recombinant PEX10 serves as a vital tool in unraveling peroxisome biology and addressing unmet medical needs in rare metabolic diseases.
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