| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PET112L |
| Uniprot No | O75879 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-557 aa |
| 活性数据 | PTGSTSNQIR GESSVAQQPL HTAQKTRKGE HKWAAVVGLE IHAQISSNSK LFSGSQVRFS APPNSLVSFF DASLPGTLPV LNRRCVEAAV MTGLALNCHI NKKSLFDRKH YFYADLPAGY QITQQRLPIA VNGSLIYGVC AGKKQSQVIP KTVRIKQIQL EQDSGKSLHD NLRSQTLIDL NRAGVGLLEV VLEPDMSCGE EAATAVRELQ LILQALGTSQ ANMAEGQLRV DANISVHHPG EPLGVRTEVK NLNSIRFLAK AIDYEIQRQI NELENGGEIL NETRSFHHKL GCTMSMRDKE GKQDYRFMPE PNLPPLVLYD ATSLPAGADP QQVINIDQIR ETLPELPSVT REKLVQQYGM LLEHSFTLLN EVGLLEFFQN VIKETRAEPK KVTSWVLNTF LGYLKQQNLA VSESPVTPSA LAELLDLLDS RTISSSAAKQ VFEELWKREG KTPGQIVSEK QLELMQDQGA LEQLCHSVME AHPQVVMDVK NRNPRAINKL IGLVRKATQS RADPVMIKEI LEKKLSL |
| 分子量 | 61.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PET112L蛋白的假设性参考文献示例(注：实际文献需根据具体研究查询数据库)：
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1. **文献名称**: *Functional characterization of recombinant human PET112L in mitochondrial translation*
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌中重组表达了人PET112L蛋白，并验证了其作为线粒体翻译辅助因子的功能。实验表明，PET112L与线粒体核糖体亚基结合，并参与细胞色素c氧化酶的生物合成。
2. **文献名称**: *Structural insights into PET112L's role in cytochrome oxidase assembly*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组人PET112L蛋白的三维结构，发现其通过保守结构域与其他线粒体复合物IV组装因子相互作用，为相关遗传性线粒体疾病的机制提供了新线索。
3. **文献名称**: *PET112L knockdown and rescue via recombinant protein in HeLa cells*
**作者**: Garcia R, et al.
**摘要**: 利用重组PET112L蛋白回补实验证明，PET112L缺陷可导致线粒体呼吸链活性下降，重组蛋白能恢复复合物IV功能，提示其在治疗线粒体疾病中的潜在价值。
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**提示**：实际研究中，PET112L可能涉及较少的直接文献。建议通过基因别名(如HGNC命名)或功能关键词(如线粒体翻译、复合物IV组装)在PubMed或UniProt中进一步检索。
**Background of Recombinant Human PET112L Protein**
The PET112L protein, encoded by the *PET112L* gene in humans, is a mitochondrial protein implicated in the biogenesis of the respiratory chain complex IV (cytochrome c oxidase). Originally identified in yeast as a translational activator for cytochrome c oxidase subunit 2 (*COX2*), its human homolog shares functional conservation, though precise molecular mechanisms remain under investigation. PET112L is believed to play a role in mitochondrial translation or assembly of oxidative phosphorylation (OXPHOS) components, crucial for cellular energy production.
Recombinant human PET112L protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to probe its interactions with mitochondrial ribosomes, COX subunits, or other assembly factors, shedding light on disorders linked to mitochondrial dysfunction, such as Leigh syndrome or encephalopathies. Mutations in *PET112L* have been associated with impaired COX activity, highlighting its clinical relevance.
Research on recombinant PET112L also aids in exploring therapeutic strategies for mitochondrial diseases, including gene therapy or small-molecule interventions targeting OXPHOS defects. Structural and biochemical analyses of the protein further contribute to understanding its role in mitochondrial gene expression and energy metabolism.
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