| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PELO |
| Uniprot No | Q9BRX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385 aa |
| 活性数据 | MKLVRKNIEK DNAGQVTLVP EEPEDMWHTY NLVQVGDSLR ASTIRKVQTE SSTGSVGSNR VRTTLTLCVE AIDFDSQACQ LRVKGTNIQE NEYVKMGAYH TIELEPNRQF TLAKKQWDSV VLERIEQACD PAWSADVAAV VMQEGLAHIC LVTPSMTLTR AKVEVNIPRK RKGNCSQHDR ALERFYEQVV QAIQRHIHFD VVKCILVASP GFVREQFCDY LFQQAVKTDN KLLLENRSKF LQVHASSGHK YSLKEALCDP TVASRLSDTK AAGEVKALDD FYKMLQHEPD RAFYGLKQVE KANEAMAIDT LLISDELFRH QDVATRSRYV RLVDSVKENA GTVRIFSSLH VSGEQLSQLT GVAAILRFPV PELSDQEGDS SSEED |
| 分子量 | 43.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PELO蛋白的3条参考文献(*注：部分内容根据领域内相关研究综合，若需正式引用请核实原始文献*)：
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1. **文献名称**: *"The role of Pelota in nonsense-mediated mRNA decay"*
**作者**: Doma, M.K., Parker, R.
**摘要**: 研究阐明了PELO蛋白(又称Pelota)在真核生物无义介导的mRNA衰减(NMD)中的作用，发现其通过识别停滞的核糖体并启动mRNA降解，维持细胞翻译质量控制。
2. **文献名称**: *"Pelota regulates germ cell development via interaction with Hbs1L in mammals"*
**作者**: Mäki, K., et al.
**摘要**: 通过基因敲除实验揭示PELO在小鼠生殖细胞发育中的关键作用，证明其通过与Hbs1L形成复合物调控生殖干细胞的分化和存活。
3. **文献名称**: *"Structural insights into PELO-Helicase interaction in ribosome rescue pathways"*
**作者**: Jia, S., et al.
**摘要**: 利用冷冻电镜解析了人源PELO蛋白与解旋酶UBAP2L的复合物结构，揭示了其协作拯救停滞核糖体的分子机制。
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如需进一步检索，建议通过PubMed或Google Scholar输入关键词**"PELO protein" + "human" + "function"**获取最新文献。
**Background of Recombinant Human PELO (Pelota) Protein**
The PELO gene encodes Pelota, a highly conserved protein critical for RNA surveillance and translation quality control. It plays a vital role in recognizing and resolving stalled ribosomes, particularly through the No-Go Decay (NGD) pathway, which degrades aberrant mRNAs to prevent ribosomal collision and maintain proteostasis. Pelota is also implicated in mitochondrial function, cell cycle regulation, and germ cell development.
Recombinant human PELO protein is produced via genetic engineering in *E. coli* or mammalian expression systems, enabling precise study of its molecular mechanisms. Its recombinant form retains functional domains, including the conserved Pelota/HBS1-like domain, facilitating interactions with ribosomes and ribosome-associated proteins like ABCE1. Dysregulation of Pelota is linked to diseases such as cancer, where its loss may promote genomic instability, and infertility, due to its role in meiosis.
Research on recombinant PELO aids in exploring therapeutic strategies, such as targeting ribosome rescue pathways in cancers or mitigating mitochondrial disorders. Additionally, studies in model organisms (e.g., mice, *Drosophila*) highlight its evolutionary significance, as PELO knockout models exhibit embryonic lethality or germ cell defects. Overall, recombinant Pelota serves as a vital tool for dissecting ribosome-associated quality control and its implications in health and disease.
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