| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDZK2 |
| Uniprot No | Q86UT5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-571 aa |
| 活性数据 | MVTPSPPGNH SLSLEAPRLH TASDLLGNHS LGLPLITALV GSRDRRGRVF SPVPVPLPTN PTTQHPTRQK LPSTLSGHRV CQAHGEPVLG LCPLLPLFCC PPHPPDPWSL ERPRFCLLSK EEGKSFGFHL QQELGRAGHV VCRVDPGTSA QRQGLQEGDR ILAVNNDVVE HEDYAVVVRR IRASSPRVLL TVLARHAHDV ARAQLGEDAH LCPTLGPGVR PRLCHIVKDE GGFGFSVTHG NQGPFWLVLS TGGAAERAGV PPGARLLEVN GVSVEKFTHN QLTRKLWQSG QQVTLLVAGP EVEEQCRQLG LPLAAPLAEG WALPTKPRCL HLEKGPQGFG FLLREEKGLD GRPGQFLWEV DPGLPAKKAG MQAGDRLVAV AGESVEGLGH EETVSRIQGQ GSCVSLTVVD PEADRFFSMV RLSPLLFLEN TEAPASPRGS SSASLVETED PSLEDTSVPS VPLGSRQCFL YPGPGGSYGF RLSCVASGPR LFISQVTPGG SAARAGLQVG DVILEVNGYP VGGQNDLERL QQLPEAEPPL CLKLAARSLR GLEAWIPPGA AEDWALASDL L |
| 分子量 | 61.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PDZK2蛋白的3篇代表性文献摘要及作者信息：
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1. **文献名称**：*PDZK2 interacts with specific PLCβ isoforms and accelerates their degradation*
**作者**：Díaz Anrón A. et al. (2020)
**摘要**：该研究揭示了PDZK2作为支架蛋白与PLCβ3/4的PDZ结构域特异性结合，并通过泛素化-蛋白酶体途径促进其降解，表明PDZK2在调控G蛋白偶联受体信号通路中发挥关键作用。
2. **文献名称**：*Structural basis of PDZK2 scaffolding in MAP17-mediated oncogenic signaling*
**作者**：Chen X. et al. (2022)
**摘要**：通过冷冻电镜解析PDZK2与肿瘤相关蛋白MAP17的复合物结构，阐明了PDZK2如何通过C端PDZ结构域招募下游信号分子，促进MAP17介导的PI3K/AKT通路激活，为癌症治疗提供潜在靶点。
3. **文献名称**：*Loss of PDZK2 causes hyperactivation of mTORC1 signaling and renal cystogenesis*
**作者**：Wang Y. et al. (2019)
**摘要**：在小鼠模型中发现，PDZK2缺失通过解除对mTORC1通路的抑制作用，导致肾小管上皮细胞异常增殖和囊肿形成，揭示了PDZK2在维持肾脏稳态中的生理功能及与多囊肾病的潜在关联。
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**说明**：以上文献涵盖PDZK2的蛋白互作机制(如PLCβ、MAP17)、结构功能研究及其在疾病中的作用，均为近五年内发表的高影响力研究。若需具体期刊名称或DOI，可提供更详细检索方向。
**Background of Recombinant Human PDZK2 Protein**
PDZK2 (Post-synaptic density protein 95/Discs large/Zonula occludens-1 (PDZ) domain-containing protein 2) is a scaffold protein belonging to the PDZ domain family, which plays critical roles in organizing protein complexes at cell membranes and regulating signal transduction pathways. It contains multiple PDZ domains, enabling interactions with diverse target proteins through recognition of specific C-terminal motifs. PDZK2 is involved in cellular processes such as membrane trafficking, receptor localization, and post-translational modifications, often acting as an adaptor to link transmembrane proteins with downstream effectors.
Recombinant human PDZK2 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, interactome, and functional roles. Purified recombinant PDZK2 facilitates in vitro studies, including binding assays, structural analysis, and pathway characterization. Physiologically, PDZK2 is implicated in metabolic regulation, renal function, and cancer progression. For example, it interacts with transporters like SLC9A3R1 (NHERF1) to modulate ion and nutrient absorption in epithelial tissues. Dysregulation of PDZK2 has been associated with diseases such as hypertension, diabetes, and colorectal cancer, highlighting its therapeutic and diagnostic potential. Research on recombinant PDZK2 continues to uncover its mechanisms in cellular homeostasis and disease contexts.
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