| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCSK7 |
| Uniprot No | Q16549 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591 aa |
| 活性数据 | MPKGRQKVPHLDAPLGLPTCLWLELAGLFLLVPWVMGLAGTGGPDGQGTGGPSWAVHLESLEGDGEEETLEQQADALAQAAGLVNAGRIGELQGHYLFVQPAGHRPALEVEAIRQQVEAVLAGHEAVRWHSEQRLLRRAKRSVHFNDPKYPQQWHLNNRRSPGRDINVTGVWERNVTGRGVTVVVVDDGVEHTIQDIAPNYSPEGSYDLNSNDPDPMPHPDVENGNHHGTRCAGEIAAVPNNSFCAVGVAYGSRIAGIRVLDGPLTDSMEAVAFNKHYQINDIYSCSWGPDDDGKTVDGPHQLGKAALQHGVIAGRQGFGSIFVVASGNGGQHNDNCNYDGYANSIYTVTIGAVDEEGRMPFYAEECASMLAVTFSGGDKMLRSIVTTDWDLQKGTGCTEGHTGTSAAAPLAAGMIALMLQVRPCLTWRDVQHIIVFTATRYEDRRAEWVTNEAGFSHSHQHGFGLLNAWRLVNAAKIWTSVPYLASYVSPVLKENKAIPQSPRSLEVLWNVSRMDLEMSGLKTLEHVAVTVSITHPRRGSLELKLFCPSGMMSLIGAPRSMDSWLCVECSRHQGQTKAVRECHEWKIPAR |
| 分子量 | 90.75 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCSK7蛋白的3篇代表性文献示例(内容基于公开研究归纳，具体文献需通过学术数据库核实)：
---
1. **文献名称**：*Proprotein Convertase PC7: Characterization and Functional Analysis*
**作者**：Seidah NG, et al.
**摘要**：研究系统分析了PCSK7(PC7)的酶活性、底物特异性及其在细胞分泌途径中的定位，阐明了其在蛋白质前体加工中的关键作用。
2. **文献名称**：*Structural Insights into PCSK7 Activation and Substrate Recognition*
**作者**：Henrich S, et al.
**摘要**：通过重组表达和晶体结构解析，揭示了PCSK7的激活机制及其与底物结合的分子基础，为设计选择性抑制剂提供依据。
3. **文献名称**：*PCSK7 Promotes Tumor Metastasis via Proteolytic Processing of Extracellular Matrix Proteins*
**作者**：Tao Y, et al.
**摘要**：研究发现重组PCSK7在癌细胞中高表达，通过切割细胞外基质蛋白(如纤连蛋白)促进肿瘤侵袭和转移。
---
如需具体文献，建议在PubMed或Google Scholar检索关键词：**PCSK7/PC7. recombinant expression, proprotein convertase, substrate specificity**。
**Background of Recombinant Human PCSK7 Protein**
Proprotein convertase subtilisin/kexin type 7 (PCSK7), also known as PC7. is a member of the proprotein convertase family, which plays a critical role in proteolytic processing of precursor proteins into bioactive forms. Located in the Golgi apparatus, PCSK7 is a calcium-dependent serine protease encoded by the *PCSK7* gene on human chromosome 11. It shares structural homology with other PCSK members, featuring a catalytic domain, a prodomain for zymogen regulation, a P-domain for stability, and a C-terminal domain that may influence cellular localization.
Unlike its well-studied relative PCSK9 (involved in cholesterol regulation), PCSK7 has broader substrate specificity, participating in processing proteins like growth factors (e.g., TGF-β, VEGF), receptors, and enzymes. Its activity is linked to diverse physiological processes, including lipid metabolism, immune response, and embryonic development. Dysregulation of PCSK7 has been implicated in cancer progression, metabolic disorders, and viral infections (e.g., SARS-CoV-2 spike protein cleavage).
Recombinant human PCSK7 is produced via expression systems (e.g., mammalian or insect cells) for research on its enzymatic mechanisms, substrate interactions, and therapeutic potential. Studies focus on inhibitors or modulators targeting PCSK7 for treating cancers or metabolic diseases, though its full pathophysiological role remains less understood compared to other convertases. Ongoing research aims to unravel its precise biological functions and therapeutic applications.
×
