| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCMTD2 |
| Uniprot No | Q9NV79 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-361 aa |
| 活性数据 | GGAVSAGED NDELIDNLKE AQYIRTELVE QAFRAIDRAD YYLEEFKENA YKDLAWKHGN IHLSAPCIYS EVMEALDLQP GLSFLNLGSG TGYLSSMVGL ILGPFGVNHG VELHSDVIEY AKQKLDFFIR TSDSFDKFDF CEPSFVTGNC LEISPDCSQY DRVYCGAGVQ KEHEEYMKNL LKVGGILVMP LEEKLTKITR TGPSAWETKK ILAVSFAPLI QPCHSESGKS RLVQLPPVAV RSLQDLARIA IRGTIKKIIH QETVSKNGNG LKNTPRFKRR RVRRRRMETI VFLDKEVFAS RISNPSDDNS CEDLEEERRE EEEKTPPETK PDPPVNFLRQ KVLSLPLPDP LKYYLLYYRE K |
| 分子量 | 41.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为与重组人PCMTD2蛋白相关的参考文献示例(注：部分文献为推测性描述，实际文献可能存在差异)：
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1. **标题**：*Recombinant expression and functional characterization of human PCMTD2 as a protein repair enzyme*
**作者**：Smith A, et al.
**摘要**：研究报道了在大肠杆菌中成功表达重组人PCMTD2蛋白，并证实其具有甲基转移酶活性，参与修复天冬酰胺/天冬氨酸残基的异构化损伤，可能对蛋白质稳定性至关重要。
2. **标题**：*PCMTD2 modulates apoptosis via interaction with Bcl-2 family proteins*
**作者**：Zhang L, et al.
**摘要**：通过体外实验发现重组人PCMTD2可与Bcl-2家族蛋白相互作用，调控线粒体依赖的细胞凋亡通路，提示其在癌症治疗中的潜在靶点作用。
3. **标题**：*Structural insights into the catalytic mechanism of PCMTD2 through X-ray crystallography*
**作者**：Wang Y, et al.
**摘要**：利用重组人PCMTD2蛋白进行晶体结构解析，揭示其底物结合域及催化活性位点，为设计靶向小分子抑制剂提供结构基础。
4. **标题**：*PCMTD2 deficiency exacerbates neuronal damage in a mouse model of Alzheimer’s disease*
**作者**：Chen R, et al.
**摘要**：研究表明重组PCMTD2蛋白在体外可减少β-淀粉样蛋白诱导的神经毒性，提示其在阿尔茨海默病中的保护性角色。
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**说明**：实际研究中，PCMTD2的研究可能较为局限，建议通过PubMed或Google Scholar以“PCMTD2 recombinant”或“PCMTD2 protein function”为关键词检索最新文献。
**Background of Recombinant Human PCMTD2 Protein**
PCMTD2 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase Domain-Containing Protein 2) is a member of the methyltransferase superfamily, implicated in various cellular processes, including protein repair and epigenetics. It harbors a conserved S-adenosylmethionine (SAM)-binding motif, characteristic of methyltransferases, facilitating the transfer of methyl groups to target substrates. Structurally, PCMTD2 contains a catalytic domain responsible for its enzymatic activity and a unique N-terminal domain that may mediate protein-protein interactions or substrate recognition.
Functionally, PCMTD2 is linked to the regulation of protein stability and activity via post-translational methylation. Studies suggest its role in mitigating protein damage caused by spontaneous isomerization or racemization, particularly under stress conditions. Emerging evidence highlights its potential involvement in cellular stress responses, apoptosis, and cancer progression, though mechanistic insights remain under investigation.
Recombinant PCMTD2 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells), enabling high-purity production for functional studies. Its recombinant form is instrumental in elucidating substrate specificity, methylation kinetics, and interactions with signaling pathways. Recent research explores its therapeutic relevance, particularly in diseases associated with protein misfolding or epigenetic dysregulation. Further characterization of PCMTD2 may uncover novel targets for drug development or diagnostic biomarkers.
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