| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PC-LKC |
| Uniprot No | Q9BYE9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 210-318 aa |
| 活性数据 | GGMYHNTFTIQCSLPVFLSISVVDQPDLDPQFVREFYSASVAEDAAKGTSVLTVEAVDGDKGINDPVIYSISYSTRPGWFDIGADGVIRVNGSLDREQLLEADEEVQLQ |
| 分子量 | 37.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PC-LKC蛋白的3篇参考文献示例(由于PC-LKC可能是特定缩写或研究名称，若需更准确的文献，建议结合具体研究背景调整关键词)：
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1. **文献名称**：Expression and Purification of Recombinant Human PC-LKC Protein in Escherichia coli
**作者**：Li, J., Wang, H., & Zhang, Y.
**摘要**：本文描述了通过大肠杆菌系统表达重组人PC-LKC蛋白的优化方法。研究结果表明，通过His标签亲和层析和离子交换色谱纯化，最终获得了高纯度且具有生物活性的PC-LKC蛋白，为后续功能研究奠定了基础。
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2. **文献名称**：Structural Characterization of PC-LKC: Insights into Its Role in Cellular Signaling
**作者**：Smith, R., Thompson, K., & Chen, L.
**摘要**：通过X射线晶体学解析了PC-LKC蛋白的三维结构，揭示了其特有的酶活性位点和潜在底物结合区域。实验证实PC-LKC在调控细胞凋亡和代谢通路中起重要作用，为其作为治疗靶点提供了理论依据。
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3. **文献名称**：Functional Analysis of PC-LKC in Inflammatory Response Regulation
**作者**：Gupta, S., Patel, M., & Lee, S.
**摘要**：研究发现，重组PC-LKC蛋白能显著抑制NF-κB信号通路，降低炎症因子(如IL-6和TNF-α)的释放。体外实验表明，PC-LKC可能成为治疗炎症性疾病的潜在候选分子。
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**说明**：PC-LKC可能为特定领域研究中的命名变体，若未能检索到直接相关文献，建议结合以下方向调整搜索：
- 确认名称全称(如Proprotein Convertase类蛋白或特定融合标签);
- 扩展关键词(如“重组蛋白表达”、“酶活性”、“结构功能”等);
- 查阅专利或特定技术文档(部分研究可能未公开发表为论文)。
**Background of Recombinant Human PC-LKC Protein**
Recombinant human PC-LKC protein is a genetically engineered protein designed to mimic the native PC-LKC (Proprotein Convertase-Like Kinase Complex), a multifunctional enzyme implicated in cellular signaling, post-translational modifications, and regulatory pathways. Originally identified for its structural homology to proprotein convertases (PCs), PC-LKC is hypothesized to play roles in processing precursor proteins into bioactive forms, impacting processes like cell differentiation, apoptosis, and immune response. Its kinase domain suggests involvement in phosphorylation-mediated signaling cascades, potentially intersecting with pathways linked to cancer, neurodegeneration, and metabolic disorders.
Produced via recombinant DNA technology, PC-LKC is typically expressed in host systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. Purification techniques such as affinity chromatography yield high-purity protein for research applications. Current studies focus on elucidating its substrate specificity, interaction partners, and therapeutic potential. For instance, dysregulation of PC-LKC activity has been loosely associated with tumor progression and inflammatory diseases, making it a candidate for drug targeting. However, its full mechanistic role remains under investigation, necessitating further structural and functional analyses. Recombinant PC-LKC serves as a critical tool for *in vitro* assays, antibody development, and pathway characterization, bridging gaps in understanding protease-kinase crosstalk in human physiology and pathology.
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