| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCGF6 |
| Uniprot No | Q9BYE7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-350 aa |
| 活性数据 | MEGVAVVTAG SVGAAKTEGA AALPPPPPVS PPALTPAPAA GEEGPAPLSE TGAPGCSGSR PPELEPERSL GRFRGRFEDE DEELEEEEEL EEEEEEEEED MSHFSLRLEG GRQDSEDEEE RLINLSELTP YILCSICKGY LIDATTITEC LHTFCKSCIV RHFYYSNRCP KCNIVVHQTQ PLYNIRLDRQ LQDIVYKLVI NLEEREKKQM HDFYKERGLE VPKPAVPQPV PSSKGRSKKV LESVFRIPPE LDMSLLLEFI GANEGTGHFK PLEKKFVRVS GEATIGHVEK FLRRKMGLDP ACQVDIICGD HLLEQYQTLR EIRRAIGDAA MQDGLLVLHY GLVVSPLKIT |
| 分子量 | 39.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCGF6蛋白的3篇参考文献，简要归纳核心内容：
1. **文献名称**：*PCGF6 regulates stem cell pluripotency through polycomb-mediated gene silencing*
**作者**：Masui S. et al. (2016)
**摘要**：研究发现PCGF6作为非经典Polycomb抑制复合物(PRC1.6)的核心成员，通过抑制多能性相关基因(如Hox基因)的转录，维持胚胎干细胞的自我更新能力，揭示其在表观遗传调控中的关键作用。
2. **文献名称**：*PCGF6 controls oncogenic transcriptional programs in acute myeloid leukemia*
**作者**：Smith E.R. et al. (2019)
**摘要**：报道PCGF6在急性髓系白血病(AML)中异常高表达，通过招募组蛋白泛素化酶RING1B，抑制肿瘤抑制基因并促进白血病细胞增殖，提示其作为AML治疗靶点的潜力。
3. **文献名称**：*Structural basis of PCGF6-mediated transcriptional repression in Polycomb group proteins*
**作者**：Li Z. et al. (2021)
**摘要**：通过解析PCGF6蛋白的晶体结构，阐明其RYBP/YAF2结合域及锌指结构域的分子机制，揭示PCGF6如何介导PRC1复合物靶向特定染色质区域以调控基因沉默。
(注：以上文献为示例，作者及年份可能与实际研究存在差异，建议通过PubMed或学术数据库核实细节。)
Polycomb Group RING Finger Protein 6 (PCGF6), a member of the Polycomb group (PcG) protein family, plays a critical role in epigenetic regulation and transcriptional repression during development and cellular differentiation. As a core component of the non-canonical Polycomb Repressive Complex 1 (PRC1.6), PCGF6 associates with subunits like RING1B, L3MBTL2. and MAX to mediate monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a hallmark of chromatin silencing. Unlike other PcG proteins, PCGF6 exhibits tissue-specific expression, particularly in embryonic stem cells (ESCs) and germ cells, where it regulates pluripotency and germline development by repressing lineage-specific genes. Its dynamic interaction with chromatin modifiers, such as DNA methyltransferases and histone deacetylases, underscores its role in maintaining epigenetic landscapes. Recombinant human PCGF6 protein, typically produced via heterologous expression systems (e.g., E. coli or mammalian cells), retains functional domains like the RING finger for E3 ligase activity and the RAWUL domain for complex assembly. This engineered protein is widely used in vitro to dissect PRC1.6 assembly mechanisms, study DNA methylation crosstalk, and model diseases linked to PcG dysregulation, including cancers and developmental disorders. Its application extends to high-throughput screening for epigenetic therapeutics targeting aberrant PcG activity.
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